Regulation of expression of IL-4 alleles:: Analysis using a chimeric GFP/IL-4 gene
Regulation of expression of IL-4 alleles:: Analysis using a chimeric GFP/IL-4 gene
复制标题
DOI：
10.1016/s1074-7613(01)00084-x
复制
发表时间：
2001-01-01
期刊：
影响因子：
32.4
通讯作者：
Paul, WE
中科院分区：
文献类型：
--
作者：
Hu-Li, J;Pannetier, C;Paul, WE
来自IL-4和GFP/IL-4基因的杂合小鼠的CD4细胞经常表达单个等位基因。从最近引发的Th2细胞中对细胞产生IL-4或GFP的分析表明，本质上所有人都有能力转录任何等位基因，但这样做的可能性较低。相比之下，在表达IL-4或GFP的细胞比例中，长期TH2克隆显示出明显且可遗传的比率。我们得出的结论是，在TH2启动早期有效事件的过程中，这两个等位基因都能被效率低下。发生的第二个事件发生了，使一个等位基因变得更有能力，这构成了不同克隆中IL-4和GFP的差异表达。
CD4 cells from mice heterozygous for an IL-4 and a GFP/IL-4 gene frequently express a single allele. Analysis of IL-4 or GFP production by cells from recently primed Th2 cells indicates that essentially all are competent to transcribe either allele but have a low probability of doing so. By contrast, long-term Th2 clones show distinct and heritable ratios in the proportion of cells that express IL-4 or GFP. We conclude that in the course of Th2 priming an early efficient event renders both alleles capable of being inefficiently transcribed; a second, less frequent event occurs that renders one allele more competent, accounting for the differential expression of IL-4 and GFP in different clones.