A Xenon-Based Molecular Sensor Assembled on an MS2 Viral Capsid Scaffold
A Xenon-Based Molecular Sensor Assembled on an MS2 Viral Capsid Scaffold
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DOI：
10.1021/ja100319f
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发表时间：
2010-05-05
影响因子：
15
通讯作者：
Pines, Alexander
中科院分区：
文献类型：
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作者：
Meldrum, Tyler;Seim, Kristen L.;Pines, Alexander
在MRI中，解剖结构最常通过其体磁特性的变化来区分。或者，外源性造影剂可以连接到赋予分子靶标亲和力的化学部分;这种造影剂的分布可以通过磁共振成像。基于氙的分子传感器是分子成像剂，其依赖于主体和特定靶向的主体-客体复合物之间的超极化氙的可逆交换。我们已经将类似于125氙传感器分子的内部的MS 2病毒衣壳，赋予多价性和其他性质的病毒衣壳的传感器分子。所产生的信号放大有助于在0.7 pM下检测传感器，这是迄今为止用于磁共振的任何分子成像剂的最低值。这种扩增保证了在比没有衣壳支架可能的低得多的浓度下检测化学靶标。
In MRI, anatomical structures are most often differentiated by variations in their bulk magnetic properties. Alternatively, exogenous contrast agents can be attached to chemical moieties that confer affinity to molecular targets; the distribution of such contrast agents can be imaged by magnetic resonance. Xenon-based molecular sensors are molecular imaging agents that rely on the reversible exchange of hyperpolarized xenon between the bulk and a specifically targeted host-guest complex. We have incorporated similar to 125 xenon sensor molecules in the interior of an MS2 viral capsid, conferring multivalency and other properties of the viral capsid to the sensor molecule. The resulting signal amplification facilitates the detection of sensor at 0.7 pM, the lowest to date for any molecular imaging agent used in magnetic resonance. This amplification promises the detection of chemical targets at much lower concentrations than would be possible without the capsid scaffold.