Regulation of myosin heavy-chain gene expression during skeletal-muscle hypertrophy.
Regulation of myosin heavy-chain gene expression during skeletal-muscle hypertrophy.
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骨骼肌肥大期间肌球蛋白重链基因表达的调节。
DOI：
10.1042/bj2570691
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Stirewalt,WS
中科院分区：
文献类型：
--
作者：
Periasamy,M;Gregory,P;Martin,BJ;Stirewalt,WS
分化肌肉的肌球蛋白表型的变化是组织适应各种生理刺激的显着特征。在本研究中，研究了协同肌去除和自发跑步运动引起的代偿性肥大期间大鼠骨骼肌中肌球蛋白同工酶比例变化的分子基础。使用针对不同 MHC 和 MLC 基因的 cDNA 探针评估大鼠跖肌（快）和比目鱼肌（慢）中肌节肌球蛋白重 (MHC) 和轻 (MLC) 链 mRNA 的相对量。特定 MHC mRNA 水平比例的变化与 mRNA 编码的肌球蛋白同工酶比例的变化方向相同，幅度相似。未检测到 MLC 蛋白或 mRNA 的比例发生显着变化。然而，在正常和肥大的比目鱼肌中均检测到高水平的 MLC3 mRNA，而肥大的比目鱼肌仅含有微量的 MLC3 蛋白。在肥大过程中，两块肌肉中都会诱导出少量的胚胎和新生儿 MHC mRNA。我们得出的结论是，骨骼肌适应超负荷工作期间肌球蛋白同工酶模式的变化是特定 MHC mRNA 水平变化的结果。
Changes in the myosin phenotype of differentiated muscle are a prominent feature of the adaptation of the tissue to a variety of physiological stimuli. In the present study the molecular basis of changes in the proportion of myosin isoenzymes in rat skeletal muscle which occur during compensatory hypertrophy caused by the combined removal of synergist muscles and spontaneous running exercise was investigated. The relative amounts of sarcomeric myosin heavy (MHC)- and light (MLC)-chain mRNAs in the plantaris (fast) and soleus (slow) muscles from rats was assessed with cDNA probes specific for different MHC and MLC genes. Changes in the proportion of specific MHC mRNA levels were in the same direction as, and of similar magnitude to, changes in the proportion of myosin isoenzymes encoded for by the mRNAs. No significant changes in the proportion of MLC proteins or mRNA were detected. However, high levels of MLC3 mRNA were measured in both normal and hypertrophied soleus muscles which contained only trace amounts of MLC3 protein. Small amounts of embryonic and neonatal MHC mRNAs were induced in both muscles during hypertrophy. We conclude that the change in the pattern of myosin isoenzymes during skeletal-muscle adaptation to work overload is a consequence of changes in specific MHC mRNA levels.