Reversed-phase high-performance liquid chromatographic identification of lutein and zeaxanthin stereoisomers in bovine retina using a C30 bonded phase
Reversed-phase high-performance liquid chromatographic identification of lutein and zeaxanthin stereoisomers in bovine retina using a C30 bonded phase
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DOI：
10.1016/s0378-4347(98)00431-9
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发表时间：
1998-12-11
期刊：
影响因子：
--
通讯作者：
Albert, K
中科院分区：
文献类型：
--
作者：
Dachtler, M;Kohler, K;Albert, K
建立了一种高效的反相高效液相色谱法，在450 nm处紫外检测，同时测定牛视网膜中叶黄素和玉米黄质的立体异构体。该程序涉及快速和小心的一步己烷提取类胡萝卜素从均质化液体和富集在线固相萃取聚苯乙烯柱上。在C-30反相柱上用丙酮-水（85.15，v/v）以1 ml/min的流速洗脱物质。与传统的C-30相相比，C-30相对类胡萝卜素立体异构体的分离表现出上级的形状选择性。为了比较，分离碘异构化叶黄素和玉米黄质标准品的混合物。(C)1998 Elsevier Science B. V.保留所有权利。
An efficient reversed-phase high-performance liquid chromatographic assay with ultraviolet detection at 450nm for simultaneous determination of lutein and zeaxanthin stereoisomers in bovine retina is described. The procedure involves rapid and careful one-step hexane extraction of the carotenoids from the homogenized liquid and enrichment by on-line solid phase extraction on a polystyrene cartridge. The substances were eluted at a flow rate of I ml/min with acetone-water (85.15, v/v) on a C-30 reversed-phase column. C-30 phases exhibit superior shape selectivity for the separation of carotenoid stereoisomers compared with conventional C-30 phases. For comparison a mixture of iodine-isomerized lutein and zeaxanthin standards was separated. (C) 1998 Elsevier Science B.V. All rights reserved.