Innervation of parasympathetic postganglionic neurons and bladder detrusor muscle directly after sacral root transection and repair using nerve transfer.
Innervation of parasympathetic postganglionic neurons and bladder detrusor muscle directly after sacral root transection and repair using nerve transfer.
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DOI：
10.1002/nau.21042
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发表时间：
2011-04
影响因子：
2
通讯作者：
Ruggieri, Michael R., Sr.
中科院分区：
文献类型：
--
作者：
Barbe, Mary F.;Ruggieri, Michael R., Sr.
这是一个延续的研究，检查膀胱神经再支配的神经根修复和神经转移的有效性。我们先前从膀胱到脊髓的逆行荧光金追踪研究发现，轴突从脊髓通过神经修复部位再生长到膀胱，这在电生理学上得到了证实。目前的研究确定了从修复的神经或神经根到膀胱的轴突再生长的模式在手术再吻合方法之间是否不同。犬膀胱去神经切断所有神经根从骶脊髓介导膀胱收缩。神经再支配手术包括端对端修复横断的骶神经前根、尾骨神经转移至骶神经前根（CG NT）或生殖股神经转移至骨盆神经（GF NT）。死后二烷基碳菁染料示踪与Neurotrace DiI从远端骨盆神经膀胱壁，结合PGP9.5神经元免疫组化，证明神经支配的DiI标记的轴突的副交感神经节后壁内神经节在正常对照组和假手术对照组，但神经支配的壁内神经节和逼尿肌直接修复后骶神经腹根。GF NT和CG NT也导致了壁内神经节和逼尿肌的神经再支配，尽管程度低于修复的神经根。膀胱神经再支配，无论是相同的神经（原位再支配）或与主要的躯体神经（尾骨）或主要的感觉神经（生殖股）的结果在再支配的壁内神经节以及逼尿肌的直接神经支配。
This is a continuation of studies examining the effectiveness of root repairs and nerve transfers for bladder reinnervation. Our previous retrograde fluorogold tracing studies from the bladder to the spinal cord found regrowth of axons from the spinal cord through the nerve repair site to the bladder which was confirmed electrophysiologically. The current study determines whether the pattern of axonal regrowth from the repaired nerves or roots to the bladder is different between the surgical reanastomosis methods. The canine bladder was denervated by transection of all nerve roots from the sacral spinal cord mediating bladder contraction. Reinnervation surgeries included end-on-end repair of transected sacral ventral roots, transfer of coccygeal to sacral ventral roots (CG NT), or transfer of genitofemoral to pelvic nerves (GF NT). Postmortem dialkylcarbocyanine dye tracing with Neurotrace DiI from the distal pelvic nerve to the bladder wall, combined with PGP9.5 neuronal immunohistochemistry, demonstrated innervation by DiI-labeled axons of only parasympathetic postganglionic intramural ganglia in normal controls and sham operated controls, but reinnervation of both intramural ganglia and detrusor muscle directly after repair of sacral ventral roots. GF NT and CG NT also resulted in reinnervation of both intramural ganglia and detrusor muscle, although to a lesser extent than repaired roots. Bladder reinnervation with either the same nerve (orthotopic reinnervation) or with either a primarily somatic nerve (coccygeal) or a primarily sensory nerve (genitofemoral) results in reinnervation of both intramural ganglia as well as direct innervation of detrusor muscle.
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DOI：
10.1038/sc.1994.52
发表时间：
1994-05-01
期刊：
PARAPLEGIA
影响因子：
--
作者：
XIAO, CG;GODEC, CJ
通讯作者：
GODEC, CJ
影响因子：
4.2
作者：
Ruggieri, Michael R., Sr.;Braverman, Alan S.;Barbe, Mary F.
通讯作者：
Barbe, Mary F.
影响因子：
6.6
作者：
Xiao, CG;Du, MX;Lepor, H
通讯作者：
Lepor, H
影响因子：
3.2
作者：
Tsai, EC;van Bendegem, RL;Tator, CH
通讯作者：
Tator, CH
影响因子：
2.2
作者：
Livshits, A;Catz, A;Gepstein, R
通讯作者：
Gepstein, R