SAPK2 contributes to rice yield by modulating nitrogen metabolic processes under reproductive stage drought stress
SAPK2 contributes to rice yield by modulating nitrogen metabolic processes under reproductive stage drought stress
复制标题
DOI：
10.1186/s12284-020-00395-3
复制
发表时间：
2020-06-08
期刊：
影响因子：
5.5
通讯作者：
Yang, Xiaoyan
中科院分区：
文献类型：
--
作者：
Lou, Dengji;Chen, Zhen;Yang, Xiaoyan
背景蔗糖非发酵1相关激酶2（SnRK 2s）在A.和水稻（Oryza sativa L.）。渗透胁迫/ABA活化蛋白激酶2（SAPK 2）是水稻SnRK 2s亚类II的成员，但其在干旱胁迫下水稻产量中的作用尚不清楚。结果与野生型（Oryza.Sativa L.spp.japonica，WT）相比，sapk 2突变体植株矮小，在生殖期干旱胁迫（RDS）下每穗粒数少，籽粒小，产量低。随后的分析表明，SAPK 2显着影响水稻籽粒的氮，磷，钾含量。对缺氮、缺钾、缺磷条件下水稻幼苗生长发育的研究表明，SAPK 2在缺氮、缺钾条件下对水稻幼苗生长和根系发育有显著影响。此外，NO3-流入速率和硝酸盐浓度分析表明，SAPK 2通过调节硝酸盐相关转运蛋白促进硝酸盐吸收和同化。结论SAPK 2基因可能通过调节氮素利用效率来提高水稻产量。本研究为培育高养分吸收的耐旱水稻品种提供了参考。
Background The sucrose non-fermenting 1-related kinases 2 (SnRK2s) play important roles in osmotic stress responses in A. thaliana and rice (Oryza sativa L.). Osmotic stress/ABA-activated protein kinase 2 (SAPK2) is a member of SnRK2s subclass II in rice, but its function in rice yield under drought stress is unclear. Results Compared with wild-type (Oryza.Sativa L.spp.japonica, WT) plants, the sapk2 rice mutant lines were shorter and produced fewer grains per panicle, smaller grains and lower grain yield under reproductive stage drought stress (RDS). Subsequent analysis suggested that SAPK2 considerably influences the nitrogen, phosphorus, and potassium contents of rice grains. The examination of rice seedling growth and development under nutrient-deprived conditions (-N, -K, and - P) proved that SAPK2 can significantly affect rice seedling growth and root development in hydroponic cultures lacking N and K. Moreover, the NO3- influx rate and nitrate concentration analysis indicated that SAPK2 promotes nitrate uptake and assimilation by regulating nitrate-related transporters. Conclusion These results suggest that SAPK2 could enhance grain production by regulating nitrogen utilization efficiency under RDS. Our work provided insights to breeding drought tolerant rice with high nutrient uptake.