INTERACTIONS OF PORPHYROMONAS-GINGIVALIS WITH EPITHELIAL-CELLS
INTERACTIONS OF PORPHYROMONAS-GINGIVALIS WITH EPITHELIAL-CELLS
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DOI：
10.1128/iai.61.5.2260-2265.1993
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发表时间：
1993-05-01
影响因子：
3.1
通讯作者：
XIE, H
中科院分区：
文献类型：
--
作者：
DUNCAN, MJ;NAKAO, S;XIE, H
某些致病性牙周细菌对牙龈上皮细胞的侵袭可能解释了它们在患病牙龈组织中的存在。为了剖析牙周病原体牙龈卟啉单胞菌潜在侵袭途径的初始步骤，对实验室和临床分离的细菌与人类口腔上皮细胞系（KB）的相互作用进行了测试。几种固定在滤过物上的牙龈假单胞菌能够结合口腔上皮细胞。定量粘附试验支持这些结果。实验测定了牙龈假单胞菌33277对上皮细胞的侵袭，透射电镜证实了该菌对上皮细胞的侵袭。这些初步结果表明，某些牙龈假单胞菌菌株能够在体外被人口腔上皮细胞内化。
The invasion of gingival epithelial cells by certain pathogenic periodontal bacteria may account for their presence within diseased gingival tissue. To dissect the initial steps of a potential invasion pathway for the periodontal pathogen Porphyromonas gingivalis, laboratory and clinical bacterial isolates were tested for their interactions with a human oral epithelial cell line (KB). Several P. gingivalis strains immobilized on filters could bind oral epithelial cells. Quantitative adherence assays supported these results. The invasion of epithelial cells by P. gingivalis 33277 was measured by assay and confirmed by transmission electron microscopy. These preliminary results demonstrate that certain P. gingivalis strains are capable of internalization by human oral epithelial cells in vitro.