A Novel Role of OS-9 in the Maintenance of Intestinal Barrier Function from Hypoxia-induced Injury via p38-dependent Pathway.
A Novel Role of OS-9 in the Maintenance of Intestinal Barrier Function from Hypoxia-induced Injury via p38-dependent Pathway.
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OS-9 通过 p38 依赖性途径维持缺氧损伤肠屏障功能的新作用
DOI：
10.7150/ijbs.10783
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发表时间：
2015
影响因子：
9.2
通讯作者：
Yang H
中科院分区：
文献类型：
--
作者：
Sun L;Xu C;Chen G;Yu M;Yang S;Qiu Y;Peng K;Wang W;Xiao W;Yang H
OS-9是内质网相关降解（ERAD）中糖基化底物的有效基化所需的一种凝集素。OS-9先前涉及er到高尔基转运和转录因子周转。然而，我们对OS-9在内质网应激下的其他功能知之甚少。本研究采用基因敲低和过表达的方法研究OS-9对肠上皮细胞Caco-2单层肠屏障功能的保护作用。我们发现OS-9通过上调occludin和claudin-1蛋白的表达，减轻了缺氧条件下肠上皮屏障功能障碍。此外，我们发现OS-9诱导occludin和claudin-1的上调是通过p38和ERK1/2磷酸化介导的，与HIF-1α无关。综上所述，我们的研究结果表明，OS-9通过激活MAP激酶（MAPK）途径上调occludin和claudin-1，从而在缺氧条件下保护Caco-2单层细胞的上皮屏障功能。
OS-9 is a lectin required for efficient ubquitination of glycosylated substrates of endoplasmic reticulum-associated degradation (ERAD). OS-9 has previously been implicated in ER-to-Golgi transport and transcription factor turnover. However, we know very little about other functions of OS-9 under endoplasmic reticulum stress. Here, we used gene knockdown and overexpression approaches to study the protective effect of OS-9 on intestinal barrier function of intestinal epithelial cell Caco-2 monolayer. We found that OS-9 attenuated intestinal epithelial barrier dysfunction under hypoxia through up-regulating occludin and claudin-1 protein expression. Furthermore, we showed that the up-regulation of occludin and claudin-1 induced by OS-9 was mediated by p38 and ERK1/2 phosphorylation and did not involve HIF-1α. In summary, our results demonstrate that OS-9 up-regulates occludin and claudin-1 by activating the MAP kinase (MAPK) pathway, and thus protects the epithelial barrier function of Caco-2 monolayer under hypoxia condition.
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影响因子：
4.6
作者：
Kim, Hee Jung;Jeong, Jae Seok;Kim, So Ri;Park, Seung Yong;Chae, Han Jung;Lee, Yong Chul
通讯作者：
Lee, Yong Chul
DOI：
10.1038/nrgastro.2010.39
发表时间：
2010-05
期刊：
Nature reviews. Gastroenterology & hepatology
影响因子：
--
作者：
通讯作者：
--
影响因子：
16
作者：
Baek, JH;Mahon, PC;Semenza, GL
通讯作者：
Semenza, GL
DOI：
10.1016/s0011-393x(03)00091-2
发表时间：
2003-06-01
影响因子：
1.9
作者：
Colak, T;Ozturk, C;Aydin, S
通讯作者：
Aydin, S
影响因子：
5.3
作者：
Li Q;Zhang Q;Wang C;Liu X;Qu L;Gu L;Li N;Li J
通讯作者：
Li J