Detection of modified amino acids in lantibiotic peptide mutacin II by chemical derivation and electrospray ionization-mass spectroscopic analysis.
Detection of modified amino acids in lantibiotic peptide mutacin II by chemical derivation and electrospray ionization-mass spectroscopic analysis.
复制标题
通过化学衍生和电喷雾电离质谱分析检测羊毛硫抗生素肽突变蛋白 II 中的修饰氨基酸。
DOI：
10.1006/abio.1996.0181
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发表时间：
1996
影响因子：
2.9
通讯作者：
Baker,J
中科院分区：
文献类型：
--
作者：
Novák,J;Kirk,M;Caufield,PW;Barnes,S;Morrison,K;Baker,J
Birmingham, Alabama 35294 tagonism technique on trypticase soy–yeast agar (TSAY) 1 as described previously (13) using S. sanguis Received November 28, 1995 Ny101 as a sensitive indicator (11). Mutacin present in solution or suspension was quantified by spotting 10 The phenotypically similar bacteria collectively ml of twofold dilutions of mutacin in phosphate-buffered known as the mutans streptococci are considered the saline onto the surface of TSAY plates overlaid with major etiologic agents responsible for dental caries. The the indicator strain (7). species most commonly associated with human disease Purification, chemical modification, and analysis of is Streptococcus mutans (1). Attempts to better under- mutacin II. Mutacin II was isolated from an overnight stand the genetic determinants which contribute to theculture of strain T8 in a series of filtration and ultrafilcariogenic potential of this organism as well as its natu-tration steps followed by selective precipitation (7). Firal history as an infectious disease have only recentlynal preparation of mutacin II was assayed for mutacin been explored in detail (2). One of the major factorsactivity and the purity was ascertained by electrospray thought to be involved with the pathogenicity of S. mu- ionization–mass spectroscopy. tans includes the ability to elaborate bacteriocin-like For detection of both thioether and didehydro amino substances, generally known as mutacins (3), which acids, the lantibiotic (10 to 30 nmol of mutacin II or may provide a selective force necessary for sustained nisin Z) was dried under vacuum and supplemented colonization in a milieu of densely packed competingwith 30 ml of derivatization mixture composed of 1.4 M organisms found in plaque (4–6).
