Hypolipidemic effects of Citrus bergamia Risso et Poiteau juice in rats fed a hypercholesterolemic diet
Hypolipidemic effects of Citrus bergamia Risso et Poiteau juice in rats fed a hypercholesterolemic diet
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DOI：
10.1021/jf071772i
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发表时间：
2007-12-26
影响因子：
6.1
通讯作者：
Trovato, Ada
中科院分区：
文献类型：
--
作者：
Miceli, Natalizia;Mondello, Maria R.;Trovato, Ada
柑橘 bergamia Risso et Poiteau 果实在卡拉布里亚（意大利）传统上被用作其降血脂特性的流行药物。采用Folin-Ciocalteu法测定佛手柑果汁总酚含量(2474.35+/-38μg/mL)；此外，HPLC 分析还鉴定出了柚皮苷 (520 ppm)、新圣草次苷 (370 ppm) 和新橙皮苷 (310 ppm)。本研究旨在探讨佛手柑汁的降血脂作用及其对高脂血症大鼠肝脏的保护作用。长期服用佛手柑（1毫升/大鼠/天）可显着降低血清胆固醇、甘油三酯和低密度脂蛋白（LDL）水平，并增加高密度脂蛋白（HDL）水平；此外，组织病理学观察表明，在接受佛手柑治疗的大鼠中，肝实质得到了保护。此外，经佛手柑处理后，粪便中性甾醇和粪便胆汁酸的排泄量有所增加。这些结果表明，C. bergamia 的降胆固醇作用可能是通过增加粪便中性甾醇和总胆汁酸排泄来介导的。除了降血脂作用外，该果汁在二苯基三硝苯肼（DPPH）测试中还显示出自由基清除活性；可能这两种效应是相关的。这些观察结果表明，积极摄入佛手柑可以通过其彻底的清除功能和降胆固醇作用来降低某些心血管疾病的风险。
Citrus bergamia Risso et Poiteau fruits have been traditionally utilized, in Calabria (Italy), as a popular remedy for their hypolipidemic properties. C. bergamia juice total phenol content (2474.35 +/- 38 mu g/ mL) was evaluated by the Folin-Ciocalteu method; moreover, HPLC analysis led to the identification of naringin (520 ppm), neoeriocitrin (370 ppm), and neohesperidin (310 ppm). The present study was designed to investigate the hypolipidemic effects of C. bergamia juice and its protective effect on liver of hyperlipidemic rats. Chronic administration of C. bergamia (1 mL/rat/day) provoked a significant reduction in serum cholesterol, triglycerides, and low-density lipoprotein (LDL) levels and an increase in high-density lipoprotein (HDL) levels; moreover, histopathological observations showed, in rats submitted to C. bergamia treatment, a protection of hepatic parenchyma. In addition, fecal neutral sterols and fecal bile acid excretion was found to be increased after C. bergamia treatment. These results suggest that the hypocholesterolemic effect of C. bergamia may be mediated by the increase in fecal neutral sterols and total bile acids excretion. In addition to the hypolipidemic effect, the juice shows radical scavenging activity in-the diphenylpicrylhydrazyl (DPPH) test; probably the two effects are related. These observations suggest that the positive intake of C. bergamia may reduce the risk of some cardiovascular diseases through its radical scavenging function and hypocholesterolemic action.