Identification of liver metastasis-related genes in a novel human pancreatic carcinoma cell model by microarray analysis
Identification of liver metastasis-related genes in a novel human pancreatic carcinoma cell model by microarray analysis
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DOI：
10.1016/j.canlet.2009.03.030
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发表时间：
2009-09-28
期刊：
影响因子：
9.7
通讯作者：
Liu, Lu-Ming
中科院分区：
文献类型：
--
作者：
Shi, Wei-Dong;Meng, Zhi-Qiang;Liu, Lu-Ming
胰腺癌肝转移预后较差，其分子机制尚不清楚。本研究采用脾内注射的方法，从人胰腺癌高转移株SW1990HM出发，对其进行亚克隆。SW1990HM细胞的体内外致瘤性、转移潜能、体外侵袭能力、细胞生长曲线、平板效率和S期细胞数均较高。SW1990HM和SW1990细胞的基因表达谱显示有40个转移相关基因表达，差异3倍。MMP10、MMP9、MMP7、CDH1、MGAT5、CTNNA1、IGF1、IL8RB、ITGA7、MDM2、MET、SSTR2、血管内皮生长因子等与肿瘤转移的发生发展有关。因此，SW1990HM是研究转移和寻找潜在治疗靶点的一个有吸引力的模型。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
Pancreatic cancer with liver metastases has a poor prognosis and the molecular mechanisms remain unclear. In this study, SW1990HM, a highly metastatic human pancreatic carcinoma line was subcloned from SW1990 by intrasplenic injection. In vivo and in vitro tumorigenicity, metastatic potential, in vitro invasion, cell growth curves, plate efficiency and S-phase cell numbers were higher in SW1990HM cells. Gene expression profiles of SW1990HM and SW1990 cells showed 40 metastasis-related genes expressed with a 3-fold difference. Thirteen of these 32.5% (13/40) were adhesion and extracellular-matrix related and twelve 30% (12/40) were cell growth and proliferation related, such as MMP10, MMP9, MMP7, CDH1, MGAT5, CTNNA1, IGF1, IL8RB, ITGA7, MDM2, MET, SSTR2 and VEGF, which were related to the onset and progression of tumor metastasis. Thus, SW1990HM is an attractive model to study metastasis and identify potential therapeutic targets. (C) 2009 Elsevier Ireland Ltd. All rights reserved.