Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs
Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs
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DOI：
10.1099/vir.0.014480-0
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发表时间：
2009-09-01
影响因子：
3.8
通讯作者：
Vahlenkamp, Thomas W.
中科院分区：
文献类型：
--
作者：
Lange, Elke;Kalthoff, Donata;Vahlenkamp, Thomas W.
目前正在引起大流行的甲型H1N1流感病毒含有源自北美和欧亚猪系祖先的基因片段。为了深入了解猪体内的病毒复制动态、临床症状和病毒传播，我们用流感病毒 A/雷根斯堡/D6/09/H1N1 鼻内感染动物。从感染后 1 天起，在接种猪的鼻拭子中检测到病毒排泄，猪一般出现轻微症状，包括发烧、打喷嚏、流鼻涕和腹泻。接触猪被感染、传播病毒并出现与接种动物相似的临床症状。所有动物的血浆样本病毒RNA均呈阴性。核蛋白和血凝素 H1 特异性抗体可在注射后 7 天通过 ELISA 检测到。 CD4(+) T 细胞在感染后立即被激活，CD4(+) 和 CD8(+) T 细胞群在注射后 3 至 7 天扩增，与临床症状相符。根据没有病毒排泄、临床症状和血清转化来判断，接触鸡仍未受到感染。
Influenza virus A/H1N1, which is currently causing a pandemic, contains gene segments with ancestors in the North American and Eurasian swine lineages. To get insights into virus replication dynamics, clinical symptoms and virus transmission in pigs, we infected animals intranasally with influenza virus A/Regensburg/D6/09/H1N1. Virus excretion in the inoculated pigs was detected in nasal swabs from 1 day post-infection (p.i.) onwards and the pigs developed generally mild symptoms, including fever, sneezing, nasal discharge and diarrhoea. Contact pigs became infected, shed virus and developed clinical symptoms similar to those in the inoculated animals. Plasma samples of all animals remained negative for virus RNA. Nucleoprotein- and haemagglutinin H1-specific antibodies could be detected by ELISA 7 days p.i. CD4(+) T cells became activated immediately after infection and both CD4(+) and CD8(+) T-cell populations expanded from 3 to 7 days p.i., coinciding with clinical signs. Contact chickens remained uninfected, as judged by the absence of virus excretion, clinical signs and seroconversion.