A single Banana streak virus integration event in the banana genome as the origin of infectious endogenous pararetrovirus
A single Banana streak virus integration event in the banana genome as the origin of infectious endogenous pararetrovirus
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DOI：
10.1128/jvi.00212-08
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发表时间：
2008-07-01
影响因子：
5.4
通讯作者：
Iskra-Caruana, Marie-Line
中科院分区：
文献类型：
--
作者：
Gayral, Philippe;Noa-Carrazana, Juan-Carlos;Iskra-Caruana, Marie-Line
植物核基因组测序揭示了广泛存在的整合病毒序列称为内源性副逆转录病毒（EPRV）。香蕉是已知的三种具有传染性EPRV的植物物种之一。香蕉（Musa balbisiana）携带香蕉条纹病毒（Banana streak virus，BSV）的整合拷贝，其通过在种间杂交中释放病毒体而具有感染性。在这里，我们分析了存在于野生二倍体M.巴尔比西亚纳通过对香蕉细菌人工染色体资源和种间遗传杂交的研究，揭示了香蕉细菌人工染色体的遗传多样性。CV. PKW含有两个类似的BSGfV的EPRV。这些整合子的基因分型及其分离模式的研究表明等位基因插入。尽管整合的BSGfV已经经历了广泛的重排，但两种EPRV都含有全长病毒基因组。整合型和游离型病毒之间的高度序列保守性表明最近的整合事件;然而，只有一个等位基因具有感染性。分析BSGfV EPRV分离的F1群体从种间遗传杂交显示，这些EPRV序列对应于两个等位基因起源于一个单一的整合事件。我们在这里描述的第一次完整的基因组和遗传组织的两个EPRV的BSGfV目前在CV。PKW应对科学家和育种家面临的挑战，以确定和产生无BSV的遗传资源。我们讨论了这种独特的宿主-病原体相互作用的遗传和基因组植物防御与战略的感染性BSGfV EPRVs的后果。
Sequencing of plant nuclear genomes reveals the widespread presence of integrated viral sequences known as endogenous pararetroviruses (EPRVs). Banana is one of the three plant species known to harbor infectious EPRVs. Musa balbisiana carries integrated copies of Banana streak virus (BSV), which are infectious by releasing virions in interspecific hybrids. Here, we analyze the organization of the EPRV of BSV Goldfinger (BSGfV) present in the wild diploid M. balbisiana cv. Pisang Klutuk Wulung (PKW) revealed by the study of Musa bacterial artificial chromosome resources and interspecific genetic cross. cv. PKW contains two similar EPRVs of BSGfV. Genotyping of these integrants and studies of their segregation pattern show an allelic insertion. Despite the fact that integrated BSGfV has undergone extensive rearrangement, both EPRVs contain the full-length viral genome. The high degree of sequence conservation between the integrated and episomal form of the virus indicates a recent integration event; however, only one allele is infectious. Analysis of BSGfV EPRV segregation among an F1 population from an interspecific genetic cross revealed that these EPRV sequences correspond to two alleles originating from a single integration event. We describe here for the first time the full genomic and genetic organization of the two EPRVs of BSGfV present in cv. PKW in response to the challenge facing both scientists and breeders to identify and generate genetic resources free from BSV. We discuss the consequences of this unique host-pathogen interaction in terms of genetic and genomic plant defenses versus strategies of infectious BSGfV EPRVs.