SCHWANN-CELL BASAL LAMINA AND NERVE REGENERATION
SCHWANN-CELL BASAL LAMINA AND NERVE REGENERATION
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DOI：
10.1016/0006-8993(83)90081-1
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发表时间：
1983-01-01
期刊：
影响因子：
2.9
通讯作者：
ONODERA, S
中科院分区：
文献类型：
--
作者：
IDE, C;TOHYAMA, K;ONODERA, S
神经节段.apprx。取7 mm长的小鼠预变性坐骨神经，反复冻融5次，杀死雪旺细胞。处理后的神经节段被移植到与近端残端接触的原始位置。分别于移植后1、2、3、5、7、10天处死动物。在移植物中部，即移植物近端远端3~4 mm和/或移植物近端和远端附近，对移植物进行EM检查。反复冷冻解冻后，用刀片将变性的神经节段切碎。切碎的神经被放置在同一神经的近端残端接触。移植后10天处死动物。在移植后1-2天内，死亡的雪旺细胞已经解体成碎片。它们逐渐被巨噬细胞吞噬。未被巨噬细胞攻击的雪旺细胞基板仍为空管(基板支架)。在接受检查的移植物中，没有雪旺细胞在冷冻和解冻过程中存活下来。再生轴突总是通过这种基板支架生长出来，与基板内表面(即原来面向雪旺细胞质膜的一侧)接触。支架外未发现轴突。移植后1~2天再生轴突与雪旺细胞无关，移植后5~7天可见雪旺细胞伴生，推测雪旺细胞可从残端沿轴突迁移。移植后10天，在移植物中部观察到的增殖的雪旺细胞已经开始分离轴突。在切碎的神经移植物中，雪旺细胞的碎裂基板重新排列成更粗的束或基板的小集合体。再生轴突无一例外地附着在这种修饰的基底板的一侧。胶原纤维与另一侧接触，表明这些修饰的基板在细胞附着方面具有与普通支架基板相同的极性。可能雪旺细胞的基板支架是生长轴突的基质，基板支架是维持再生轴突的有效途径。支架的内表面可能有一些特定的物质负责支持再生的轴突。
Nerve segments .apprx. 7 mm long were excised from the predegenerated sciatic nerves of mice, and treated 5 times by repetitive freezing and thawing to kill the Schwann cells. The treated nerve segments were grafted into the original places in contact with the proximal stumps. The animals were sacrificed 1, 2, 3, 5, 7 and 10 days after the grafting. The grafts were examined by EM in the middle part of the graft, i.e., 3-4 mm distal to the proximal end and/or near the proximal and distal ends of the graft. The predegenerated nerve segments were minced with a razor blade after repetitive freezing and thawing. Minced nerves were placed in contact with the proximal stumps of the same nerves. The animals were sacrificed 10 days after the grafting. Within 1-2 days after grafting, the dead Schwann cells had disintegrated into fragments. They were gradually phagocytosed by macrophages. The basal laminae of Schwann cells, which were not attacked by macrophages, remained empty tubes (basal lamina scaffolds). In the grafts examined, no Schwann cells survived the freezing and thawing process. The regenerating axons always grew out through such basal lamina scaffolds, being in contact with the inner surface of the basal lamina, (i.e., the side originally facing the Schwann cell plasma membrane). No axons were found outside of the scaffolds. The regenerating axons were not associated with Schwann cells 1-2 days after grafting, but after 5-7 days they were accompanied by Schwann cells which were presumed to be migrating along axons from the proximal stumps. Ten days after grafting, proliferating Schwann cells observed in the middle part of the grafts had begun to sort out axons. In the grafts of minced nerves, the fragmented basal laminae of the Schwann cells re-arranged themselves into thicker strands or small aggregations of basal laminae. The regenerating axons, without exception, attached to one side of such modified basal laminae. Collagen fibrils were in contact with the other side, indicating that these modified basal laminae had the same polarity in terms of cell attachment as seen in the ordinary basal laminae of the scaffolds. Probably, basal lamina scaffolds of Schwann cells function as the substrate for growing axons, and that the basal lamina scaffolds are effective pathways for the maintenance of regenerating axons. The inner surface of the scaffold might have some specific substances responsible for supporting the regenerating axons.