Ulex europaeus 1 lectin targets microspheres to mouse Peyer's patch M-cells in vivo
Ulex europaeus 1 lectin targets microspheres to mouse Peyer's patch M-cells in vivo
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DOI：
10.1016/s0264-410x(97)00222-3
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发表时间：
1998-03-01
期刊：
影响因子：
5.5
通讯作者：
Hirst, BH
中科院分区：
文献类型：
--
作者：
Foster, N;Clark, MA;Hirst, BH
在微球涂覆多种试剂后，在小鼠肠袢模型中研究了 Inter 微球与小鼠派尔氏集结膜 M 细胞的相互作用。羧化微球（直径0.5μm）共价包被有凝集素Ulex europaeus 1、Concanavalin A、Euonymus europaeus和Bandeiraea simplicifolia 1 isolectin-B-4、人免疫球蛋白A或牛血清白蛋白。在所检查的处理中，只有 Ulex europaeus (UEA1) 导致微球与 M 细胞显着选择性结合。 UEA1 包被的微球与 M 细胞的结合水平是 BSA 包被的微球的 100 倍，但与肠细胞的结合不受影响。 UEA1 包被的微球与 chi-L-岩藻糖的孵育将 M 细胞的结合降低到与 BSA 包被的微球相当的水平。这表明 UEA1 的靶向是通过 M 细胞表面的碳水化合物受体进行的。 UEA1包被的微球在接种到小鼠肠环后10分钟内发生对M细胞的粘附，并且UEA1包被的微球在接种后60分钟内被运输到M细胞顶端表面以下10μm处。胃内给药后，UEA1 包被的微球还靶向小鼠派尔氏淋巴集结 M 细胞。这些结果表明，改变羧化聚苯乙烯微球的表面化学可以增加 M 细胞的靶向性，这表明了一种增强疫苗抗原向粘膜免疫系统的递送的策略。 (C) 1998 Elsevier Science Ltd. 保留所有权利。
The interaction of Inter microspheres with mouse Peyer's patch membranous M-cells was studied in a mouse gut loop model after the microspheres were coated with a variety of agents. Carboxylated microspheres (diameter 0.5 mu m) were covalently coated with lectins Ulex europaeus 1, Concanavalin A, Euonymus europaeus and Bandeiraea simplicifolia 1 isolectin-B-4, human immunoglobulin A or bovine serum albumin. Of the treatments examined, only Ulex europaeus (UEA1) resulted in significant selective binding of microspheres to M-cells. UEA1-coated microspheres bound to M-cells at a level 100-fold greater than BSA-coated microspheres, but binding to enterocytes was unaffected Incubation of UEA1-coated microspheres with chi-L-fucose reduced M-cell binding to a level comparable with BSA-coated microspheres. This indicated that targeting by UEA1 was via a carbohydrate receptor on the M-cell surface. Adherence of UEA1-coated microspheres to M-cells occurred within 10 min of inoculation into mouse gut loops and UEA1-coated microspheres were transported to 10 mu m below the apical surface of M-cells within 60 min of inoculation. UEA1-coated microspheres also targeted mouse Peyer's patch M-cells after intragastric administration. These results demonstrated that altering the surface chemistry of carboxylated polystyrene microspheres increased M-cell targeting, suggesting a strategy to enhance delivery of vaccine antigens to the mucosal immune system. (C) 1998 Elsevier Science Ltd. All rights reserved.