Plasma surface modification of electrospun fibers for adhesion-based cancer cell sorting
Plasma surface modification of electrospun fibers for adhesion-based cancer cell sorting
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DOI：
10.1039/c2ib20025b
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发表时间：
2012-01-01
影响因子：
2.5
通讯作者：
Powell, H. M.
中科院分区：
文献类型：
--
作者：
Blackstone, B. N.;Willard, J. J.;Powell, H. M.
个性化癌症治疗推动了对能够快速准确地将癌细胞与实体瘤分离的设备的需求。一种潜在的分选策略是根据细胞的相对粘附强度来分离细胞群。为了研究表面亲水性和细胞表型对粘附的影响，将原代人乳房皮肤成纤维细胞和角质形成细胞以及MCF-7乳腺癌细胞接种到空气和CF4等离子体处理的纳米纤维上，然后在接种1小时后暴露于三种剪切应力（200、275和350达因/cm(2)）。在任一等离子体处理表面上的成纤维细胞或角质形成细胞中均未测量到粘附强度的差异：在暴露于 350 达因/cm(2) 的剪切应力 5 分钟后，所有细胞都表现出 > 60% 的初始细胞计数。相比之下，MCF-7 细胞在空气和 CF4 等离子体处理表面上的相对粘附强度之间存在显着差异：分别有 26% 和 6.6% 的细胞保留在空气和 CF4 等离子体处理表面上。通过将所有三种细胞类型的混合物同时接种到 CF4 处理的纳米纤维上，然后培养 1 小时并暴露于 350 达因/厘米 (2) 剪切应力，评估从两种非癌性原代人类细胞中分选这种癌细胞系的能力。大多数 MCF-7 细胞被去除（保留 0.7%），而大多数成纤维细胞和角质形成细胞仍然粘附（74% 和 57%）。分选后的 MCF-7 活力和形态保持不变，保留了后分离和分析的可能性。这些数据表明，电纺支架的等离子体处理提供了一种可用于根据粘附强度从混合细胞群中分选癌细胞的工具。
Personalized cancer therapies drive the need for devices that rapidly and accurately segregate cancer cells from solid tumors. One potential sorting strategy is to segregate populations of cells based on their relative strength of adhesion. To investigate the effect of surface hydrophilicity and cell phenotype on adhesion, primary human breast skin fibroblasts and keratinocytes and MCF-7 breast cancer cells were seeded onto air and CF4 plasma-treated nanofibers followed by exposure to three shear stresses (200, 275 and 350 dynes per cm(2)) 1 hour after inoculation. No difference in strength of adhesion was measured in either fibroblasts or keratinocytes on either plasma treated-surface: all exhibited >60% of the initial cell count after a 5 minute exposure to 350 dynes per cm(2) of shear stress. In contrast, a significant difference between relative strength of adhesion on air versus CF4 plasma-treated surfaces was observed for MCF-7 cells: 26% and 6.6% of cells remained on the air and CF4 plasma-treated surfaces, respectively. The ability to sort this cancer cell line from two non-cancerous primary human cells was evaluated by inoculating a mixture of all three cell types simultaneously onto CF4 treated nanofibers followed by 1 hour of culture and exposure to 350 dynes per cm(2) shear stress. The majority of MCF-7 cells were removed (0.7% remained) while a majority of fibroblasts and keratinocytes remained adhered (74 and 57%). Post-sorted MCF-7 viability and morphology remained unchanged, preserving the possibility of post-separation and analysis. These data suggest that the plasma treatment of electrospun scaffolds provides a tool useful in sorting cancer cells from a mixed cell population based on adhesion strength.