Immunolocalization of DMP1 and sclerostin in the epiphyseal trabecule and diaphyseal cortical bone of osteoprotegerin deficient mice
Immunolocalization of DMP1 and sclerostin in the epiphyseal trabecule and diaphyseal cortical bone of osteoprotegerin deficient mice
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DOI：
10.2220/biomedres.31.307
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发表时间：
2010-10-01
影响因子：
1.2
通讯作者：
Amizuka, Norio
中科院分区：
文献类型：
--
作者：
Masuki, Hideo;Li, Minqi;Amizuka, Norio
为了明确骨细胞在加速骨重建中的作用，我们研究了骨细胞陷窝-管系统(OLCS)和骨细胞分泌分子牙本质基质蛋白(DMP)1和硬化素-1在骨保护素缺乏(OPG(-/-))小鼠的骨痂和皮质骨中的分布。而OPG(-/-)小鼠的骨痂和皮质骨中有排列整齐的小管，而OPG(-/-)小鼠的骨痂中有排列整齐的小管。DMP1阳性的骨细胞均匀分布于野生型骨痂和皮质骨以及OPG(-/-)皮质骨。然而，OPG(-/-)骨痂显示出弱的DMP1免疫反应。因此，随着OLCS变得规则，骨细胞似乎合成了更多的DMP1。相反，硬化素免疫反应在OPG(-/-)骨痂和皮质骨中显著减少。在OPG(-/-)骨痂和皮质骨中，三重染色显示在厚厚的碱性磷酸酶阳性的成骨细胞层的外围有少量硬化素阳性的骨细胞和许多抗酒石酸酸性磷酸酶阳性的破骨细胞。综上所述，OLCs的规律性分布可能会影响DMP1的合成，而破骨细胞和成骨细胞的细胞活动而不是OLCs的规律性最终可能会影响硬化素的合成。
In order to define the osteocytic function in accelerated bone remodeling, we examined the distribution of the osteocytic lacunar-canalicular system (OLCS) and osteocyte-secreting molecules dentin matrix protein (DMP) 1 and sclerostin-in the epiphyses and cortical bones of osteoprotegerin deficient (OPG(-/-)) mice. Silver impregnation visualized a well-arranged OLCS in the wild-type epiphyses and cortical bone, whereas OPG(-/-) mice had an irregular OLCS in the epiphyses, but well-arranged canaliculi in the cortical bone. DMP1-positive osteocytes were evenly distributed throughout the wild-type epiphyses and cortical bone, as well as the OPG(-/-) cortical bone. However, OPG(-/-) epiphyses revealed weak DMP1-immunoreactivity. Thus, osteocytes appear to synthesize more DMP1 as the OLCS becomes regular. In contrast, sclerostin-immunoreactivity was significantly diminished in the OPG(-/-) epiphyses and cortical bone. In OPG(-/-) epiphyses and cortical bone, triple staining demonstrated few sclerostin-positive osteocytes in the periphery of a thick cell layer of alkaline phosphatase-positive osteoblasts and many tartrate resistant acid phosphatase-positive osteoclasts. Summarizing, the regular distribution of OLCS may affect DMP1 synthesis, while the cellular activities of osteoclasts and osteoblasts rather than the regularity of OLCS may ultimately influence sclerostin synthesis.