The MADS-box transcription factor CmAGL11 modulates somatic embryogenesis in Chinese chestnut (Castanea mollissima Blume)
The MADS-box transcription factor CmAGL11 modulates somatic embryogenesis in Chinese chestnut (Castanea mollissima Blume)
复制标题
MADS-box转录因子CmAGL11调节板栗体细胞胚胎发生
DOI：
10.1016/s2095-3119(20)63157-4
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发表时间：
2020-04-01
影响因子：
4.8
通讯作者：
Qin Ling
中科院分区：
文献类型：
--
作者：
Gao Yue-rong;Sun Jia-chen;Qin Ling
体细胞胚胎发生(SE)是依赖于植物细胞全能性的一种有效的体外再生方法。然而，板栗(Castanea Mollissima Blume)等木本植物中SE的分子机制尚不清楚，限制了木本植物产业的发展。在此，我们报道了板栗MADS-box转录因子CmAGL11。CmAGL11转录本在球状胚胎中特异积累。CmAGL11在板栗愈伤组织中的过表达增强了其SE能力，体细胞胚胎的发育速度明显快于对照。RNA-SEQ结果表明，CmAGL11影响几个与赤霉素、生长素和乙烯途径相关的基因的表达。此外，DNA甲基化状态的分析表明，启动子甲基化在SE过程中对CmAGL11的表达起着调节作用。我们的结果表明，CmAGL11可能通过调节赤霉素、生长素和乙烯途径，在板栗的SE过程中发挥重要作用。这将有助于为加快板栗遗传改良和种质创新建立一个高效的平台。
Somatic embryogenesis (SE) is an effective approach of in vitro regeneration that depends on plant cell totipotency. However, largely unknown of molecular mechanisms of SE in woody plants such as Chinese chestnut (Castanea mollissima Blume), limits the development of the woody plant industry. Here, we report the MADS-box transcription factor CmAGL11 in Chinese chestnut. CmAGL11 transcripts specifically accumulated in the globular embryo. Overexpression of CmAGL11 in chestnut callus enhanced its SE capacity, and the development of somatic embryos occurred significantly faster than in the control. RNA-seq results showed that CmAGL11 affects the expression of several genes related to the gibberellin, auxin, and ethylene pathways. Moreover, the analysis of DNA methylation status indicated that the promoter methylation plays a role in regulation of CmAGL11 expression during SE. Our results demonstrated that CmAGL11 plays an important role in the SE process in Chinese chestnut, possibly by regulating gibberellin, auxin, and ethylene pathways. It will help establish an efficient platform to accelerate genetic improvement and germplasm innovation in Chinese chestnut.