Hydrophobic proteins of lamellated osmiophilic bodies isolated from pig lung.
Hydrophobic proteins of lamellated osmiophilic bodies isolated from pig lung.
复制标题
从猪肺中分离出的层状嗜锇小体的疏水蛋白。
DOI：
10.1042/bj1830731
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
G. E. Newman
中科院分区：
文献类型：
--
作者：
P. Phizackerley;M. Town;G. E. Newman
1. 除了不溶于有机溶剂的蛋白质外，从猪肺中分离的层状体和从支气管肺灌洗液中制备的表面活性剂含有另一组蛋白质，它们与脂质一起被提取到有机相中。2. 这些疏水蛋白约占层状体总蛋白的40%，约占灌洗液表面活性剂总蛋白的13%，而有机溶剂提取的猪肺微粒体部分和线粒体总蛋白不到1%。3. 层析方法将层状体的疏水蛋白分成四部分，并将其从磷脂中分离出来。它们的表观分子量在11500到16500之间，它们含有72- 79%的疏水残基和16- 22%的含硫氨基酸，亮氨酸是每种情况下主要的n端氨基酸。
1. In addition to proteins that are insoluble in organic solvents, lamellated bodies isolated from pig lung and surfactant prepared from bronchopulmonary lavage fluid contain another group of proteins that are extracted together with lipid into the organic phase. 2. These hydrophobic proteins constitute about 40% of the total protein of lamellated bodies and about 13% of the total protein of surfactant isolated from lavage fluid, whereas less than 1% of the total protein of pig lung microsomal fraction and mitochondria is extracted by organic solvents. 3. The hydrophobic proteins of lamellated bodies were separated into four fractions and freed from phospholipid by chromatographic procedures. Their apparent molecular weights vary between 11 500 and 16 500, they contain 72--79% of hydrophobic residues and 16--22% of sulphur-containing amino-acids, and leucine is the major N-terminal amino acid in each case.