Anti-mastitis SNV identification of NFκB1 in Chinese Holstein cows and the possible anti-inflammation role of NFκB1/p105 in bovine MECs
Anti-mastitis SNV identification of NFκB1 in Chinese Holstein cows and the possible anti-inflammation role of NFκB1/p105 in bovine MECs
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DOI：
10.1093/abbs/gmaa118
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发表时间：
2020-11-01
影响因子：
3.7
通讯作者：
Liu, Xiaolin
中科院分区：
文献类型：
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作者：
Chen, Ling;Tian, Rongfu;Liu, Xiaolin
NF kappa B1/p105是NF kappa B家族的关键成员，当p50/p50同型二聚体形成时，NF kappa B1/p105可以抑制炎症、衰老和癌症。目前，关于NF κ B1/p105在奶牛乳腺炎中的作用的研究有限。本研究以547头中国荷斯坦奶牛为研究样本，分析了NF κ B1基因的6个单核苷酸变异与体细胞计数、产奶量、乳脂含量和乳蛋白含量的相关性，并通过转录组测序技术研究了NF κ B家族和泛素连接酶（β TrCP1、β TrCP2、KPC1、KPC2）在脂多糖诱导的牛乳腺上皮细胞（MECs）中的mRNA表达谱。相关性分析表明，NF κ B1基因SNV2-TT和snv2 - cc奶牛的乳蛋白含量显著高于对照组(P
NF kappa B1/p105 is the critical member of the NF kappa B family which can suppress inflammation, ageing, and cancer when p50/p50 homodimer is formed. Currently, the research about the role of NF kappa B1/p105 during cow mastitis is limited. Here, we analyzed the correlation of six single-nucleotide variants of the NF kappa B1 gene with somatic cell count, milk yield, milk fat content, and milk protein content in 547 Chinese Holstein cows, and explored the mRNA expression profiles of the NF kappa B family and ubiquitin ligases (beta TrCP1, beta TrCP2, KPC1, KPC2) in LPS-induced bovine mammary epithelial cells (MECs) by transcriptome-Seq. The association analysis showed that cows with SNV2-TT and SNV6-CC in the NF kappa B1 gene had significantly higher milk protein content (P