Native and sulfated oligoglucuronans as elicitors of defence-related responses inducing protection against Botrytis cinerea of Vitis vinifera
Native and sulfated oligoglucuronans as elicitors of defence-related responses inducing protection against Botrytis cinerea of Vitis vinifera
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DOI：
10.1016/j.carbpol.2011.09.084
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发表时间：
2012-01-15
影响因子：
11.2
通讯作者：
Petit, Emmanuel
中科院分区：
文献类型：
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作者：
Caillot, Sebastien;Rat, Stephanie;Petit, Emmanuel
在过去的二十年中，β -(1,4)- d -聚葡萄糖醛酸（葡糖醛酸）的物理化学和生物学特性在不同的模型中被大量描述。在这项研究中，一种部分乙酰化的细菌葡萄糖醛酸被真菌葡萄糖醛酸裂解酶降解，得到不饱和低聚葡萄糖醛酸，最后测试了它们作为葡萄天然防御的激发子的潜力。目的是利用不同程度聚合、乙酰化和磺化的低聚葡糖醛酸，将生物活性与特定的结构特征联系起来。结果表明，这些阴离子低聚物引起的不同防御反应取决于聚合程度和取代基（乙酰基或硫酸盐基）的性质。聚合度为10度和14度的乙酰化低聚葡聚糖诱导H2O2的瞬时产生和一些防御相关基因（PAL、STS、Chit4c和PG1P）的表达。聚合度为14的乙酰化低聚葡萄糖醛酸处理后，葡萄叶灰霉病的侵染率降低了23%。硫代低聚葡萄糖醛酸对H2O2的产生无效，但诱导了其他防御相关的PIN、PGIP基因的强烈表达。这项工作为保护葡萄球菌和其他植物免受病原体侵害开辟了新的和有价值的策略。(C) 2011 Elsevier Ltd.版权所有。
During the past two decades, beta-(1,4)-D-polyglucuronic acids (glucuronans) have been intensively described in literature for their physico-chemical and biological properties in different models. In this study, a bacterial glucuronan partially acetylated was degraded by a fungal glucuronan lyase to obtain unsaturated oligogluguronans, finally tested for their potential as elicitors of natural defences in Vitis vinifera. The objective was to correlate biological activities with specific structural features using oligoglucuronans varying in degree of polymerization, acetylation and sulfation. The results demonstrated that different defence reactions elicited by these anionic oligomers were dependent on degree of polymerization and nature of substituents (acetyl or sulfate groups). Acetylated oligoglucuronans with degrees of polymerization of 10 and 14 induced transient production of H2O2 and expression of some defence-related genes (PAL, STS,Chit4c and PG1P). The infection of grapevine leaves by Botrytis cinerea was reduced (23%) after treatment with acetylated oligoglucuronans having degree of polymerization of 14. Sulfated oligoglucuronans were not effective for production of H2O2 but induced strong expression of other defence-related PIN, PGIP genes. This work opens the way to new and valuable strategies to protect V. vinifera and other plants against pathogens. (C) 2011 Elsevier Ltd. All rights reserved.