Structure determination of Streptococcus suis serotype 2 capsular polysaccharide
Structure determination of Streptococcus suis serotype 2 capsular polysaccharide
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DOI：
10.1139/o09-170
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发表时间：
2010-06-01
影响因子：
2.9
通讯作者：
Gottschalk, Marcello
中科院分区：
文献类型：
--
作者：
Van Calsteren, Marie-Rose;Gagnon, Fleur;Gottschalk, Marcello
对猪链球菌2型荚膜多糖进行了分离、纯化、化学修饰和表征。CPS的糖和绝对构型分析给出以下组成：D-Gal，3; D-Glc，1; D-GlcNAc，I; D-Neu 5Ac。1; L-Rha，I.唾液酸被认为是终端，和CPS定量脱唾液酸化温和的酸水解。CPS也进行高碘酸盐氧化，然后进行硼氢化物还原和Smith降解。天然CPS或其特异性修饰产物的糖和甲基化分析、H-1和C-13核磁共振和质谱分析允许确定重复单元序列：[4）[Neu5Ac（α 2-6）半乳糖（β 1-4）GlcNAc（β 1-3）]Gal（测试版1-4）[加尔（α 1-3）]Rha（β 1-4）葡萄糖（β 1-]（n）发现骨架序列与无乳链球菌或B族链球菌（GBS）的骨架序列相同VIII型和肺炎链球菌23 F型。色葡萄猪CPS与Ia、I、II、III和IV型GBS CPS共有序列Neu 5Ac-Gal-GlcAc-Gal，但与它们的不同之处在于存在鼠李糖，并且唾液酸是2，6-而不是2，3-连接到随后的Gal。S.猪CPS序列和血清型c2 cps基因座编码的推定酶负责的重复单位的生物合成基因的初步建立。
The capsular polysaccharide (CPS) of Streptococcus suis serotype 2 was isolated, purified, chemically modified, and characterized. Sugar and absolute configuration analyses of the CPS gave the following composition: D-Gal, 3; D-Glc, 1; D-GlcNAc, I; D-Neu5Ac. 1; L-Rha, I. Sialic acid was found to be terminal, and the CPS was quantitatively desialylated by mild acid hydrolysis. The CPS was also submitted to periodate oxidation followed by borohydride reduction and Smith degradation. Sugar and methylation analysis, H-1 and C-13 nuclear magnetic resonance, and mass spectrometry of the native CPS or of its specifically modified products allowed to determine the repeating unit sequence: [4)[Neu5Ac(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-3)]Gal(beta 1-4)[Gal(alpha 1-3)]Rha(beta 1-4)Glc(beta 1-](n) The backbone sequence was found to be identical to that of Streptococcus agalactiae or group B Streptococcus (GBS) type VIII and Streptococcus pneumoniae type 23F. The S. suis CPS shares the sequence Neu5Ac-Gal-GlcAc-Gal in common with GBS types la, I, II, Ill, and IV CPSs but differs from them by the presence of rhamnose and the fact that sialic acid is 2,6- rather than 2,3-linked to the following Gal. A correlation between the S. suis CPS sequence and genes of the serotypc 2 cps locus encoding putative enzymes responsible for the biosynthesis of the repeating unit was tentatively established.