Macrophage-secreted myogenic factors: a promising tool for greatly enhancing the proliferative capacity of myoblasts in vitro and in vivo
Macrophage-secreted myogenic factors: a promising tool for greatly enhancing the proliferative capacity of myoblasts in vitro and in vivo
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DOI：
10.1007/s100720200060
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发表时间：
2002-10-01
影响因子：
3.3
通讯作者：
Vitiello, L
中科院分区：
文献类型：
--
作者：
Cantini, M;Giurisato, E;Vitiello, L
在这项工作中，我们着手确定，如果鼠巨噬细胞J774细胞系可以用来生产生肌生长因子。活化的J774巨噬细胞在无血清条件下生长。然后用巨噬细胞条件培养基（MCM）分别在体外和体内处理原代成肌细胞和再生肌组织培养物。通过分析肌肉特异性转录因子的表达，与细胞的增殖和分化速率平行，测试MCM体外活性。巨噬细胞分泌的因子大大增强了大鼠和人的原代成肌细胞的增殖潜力，并被发现是高度肌肉特异性的。在体内，MCM管理显着增强损伤肌肉的再生过程。在不存在血清的情况下产生大量巨噬细胞分泌的生肌因子的能力对于其生物化学表征和在治疗方案中的连续应用（用于离体基因治疗和肌肉修复）具有很大的希望。
In this work we set out to determine if the murine macrophage J774 cell line can be used to produce myogenic growth factors. Activated J774 macrophages were grown in serum-free conditions. The macrophage-conditioned medium (MCM) was then used to treat cultures of primary myoblasts and regenerating muscle tissue, in vitro and in vivo respectively. MCM activity in vitro was tested by analyzing the expression of muscle-specific transcription factors, in parallel with the proliferation and differentiation rates of the cells. The macrophage-secreted factors greatly enhanced the proliferative potential of both rat and human primary myoblasts and were found to be highly muscle-specific. In vivo, MCM administration markedly enhanced the regenerative processes in damaged muscles. The ability to produce large amounts of macrophage-secreted myogenic factor(s) in the absence of serum holds great promise for its biochemical characterization and successive application in therapeutic protocols, both for ex vivo gene therapy and for muscle repair.