Mechanism of initiation of transcription by Bacillus subtilis RNA polymerase at several promoters.
Mechanism of initiation of transcription by Bacillus subtilis RNA polymerase at several promoters.
复制标题
枯草芽孢杆菌 RNA 聚合酶在几个启动子处启动转录的机制。
DOI：
10.1016/0022-2836(92)90660-c
复制
发表时间：
1992
影响因子：
5.6
通讯作者：
Sonenshein,AL
中科院分区：
文献类型：
--
作者：
Whipple,FW;Sonenshein,AL
The behavior of the major vegetative cell RNA polymerase ofBacillus subtilis, EσA, during initiation of transcription was compared to that of itsEscherichia colicounterpart, Eσ70, at several promoters known to be actively transcribed by both RNA polymerases. Challenge experiments using heparin, restriction endonucleases, and competing promoter DNA under various conditions showed that, at several promoters, complexes withB. subtilisRNA polymerase formed in the absence of nucleoside triphosphates were unstable. These complexes produced DNase I footprints that were less extended than those produced by theE. colienzyme at the same promoters. Further, in the presence of certain combinations of nucleoside triphosphates, conditions that allow production of abortive oligonucleotides, theseB. subtilisRNA polymerase complexes remained dissociable. Thus, at these promoters, theB. subtilisenzyme interacted with the DNA and reached a catalytically active initial transcribing complex without becoming committed to the template. At these same promoters,E. coliRNA polymerase formed stable open complexes before forming any phosphodiester bonds.B. subtilisinitial transcribing complexes also remained sensitive to the drug rifampicin until a later stage in the initiation process than did the correspondingE. colicomplexes. At one promoter,B. subtilisEσAandE. coliEσ70behaved similarly, forming stable open complexes in the absence of any nucleoside triphosphates.
