Evaluation of bat adenoviruses suggests co-evolution and host roosting behaviour as drivers for diversity.
Evaluation of bat adenoviruses suggests co-evolution and host roosting behaviour as drivers for diversity.
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DOI：
10.1099/mgen.0.000561
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发表时间：
2021-04
影响因子：
3.9
通讯作者：
Lange CE
中科院分区：
文献类型：
--
作者：
Ntumvi NF;Diffo JLD;Tamoufe U;Ndze VN;Takuo JM;Mouiche MMM;Nwobegahay J;LeBreton M;Gillis A;Rimoin AW;Schneider BS;Monagin C;McIver DJ;Joly DO;Wolfe ND;Rubin EM;Lange CE
腺病毒（AdV）是人类和动物的多种病原体，已经鉴定了几十种蝙蝠AdV。考虑到已知的人类AdV超过100种，以及蝙蝠物种的巨大多样性，许多蝙蝠AdV可能仍未被发现。为了了解更多关于AdV的流行，多样性和进化，我们在喀麦隆使用几种病毒和宿主DNA的PCR检测对蝙蝠进行了采样和测试。在属于37个不同蝙蝠物种的671只采样动物中，有14%检测到腺病毒DNA。 有物种栖息在较大的群体和腺病毒DNA检测之间的相关性。检测到的AdV DNA属于28至44种不同的，大多数是以前未知的，mastadenovirus物种。新的分离株在遗传学上是多样的，虽然一些与已知病毒聚类，但其他分离株似乎形成了不同的新簇。新的和以前已知的蝙蝠腺病毒的系统发育树并不反映的各种宿主物种，但确实包含的结构与一定程度的病毒宿主的共同进化。考虑到在不同的宿主物种中发现了密切相关的分离株，似乎至少有一些蝙蝠腺病毒跨越了物种屏障，可能是在最近的过去;然而，该树也与整个蝙蝠腺病毒进化过程中发生的此类事件一致。AdV多样性最高的蝙蝠物种栖息在大集团。该研究显着增加了已知由蝙蝠窝藏的AdV的多样性，并表明宿主行为，如栖息大小，可能是将一些AdV限制在一个物种上的原因，而不是AdV无法感染其他相关宿主。
Adenoviruses (AdVs) are diverse pathogens of humans and animals, with several dozen bat AdVs already identified. Considering that over 100 human AdVs are known, and the huge diversity of bat species, many bat AdVs likely remain undiscovered. To learn more about AdV prevalence, diversity and evolution, we sampled and tested bats in Cameroon using several PCR assays for viral and host DNA. AdV DNA was detected in 14 % of the 671 sampled animals belonging to 37 different bat species. There was a correlation between species roosting in larger groups and AdV DNA detection. The detected AdV DNA belonged to between 28 and 44 different, mostly previously unknown, mastadenovirus species. The novel isolates are phylogenetically diverse and while some cluster with known viruses, others appear to form divergent new clusters. The phylogenetic tree of novel and previously known bat AdVs does not mirror that of the various host species, but does contain structures consistent with a degree of virus–host co-evolution. Given that closely related isolates were found in different host species, it seems likely that at least some bat AdVs have jumped species barriers, probably in the more recent past; however, the tree is also consistent with such events having taken place throughout bat AdV evolution. AdV diversity was highest in bat species roosting in large groups. The study significantly increased the diversity of AdVs known to be harboured by bats, and suggests that host behaviours, such as roosting size, may be what limits some AdVs to one species rather than an inability of AdVs to infect other related hosts.