RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation.
RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation.
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DOI：
10.1126/science.1211651
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发表时间：
2012-01-06
期刊：
影响因子：
--
通讯作者：
Grewal SI
中科院分区：
文献类型：
--
作者：
Zofall M;Yamanaka S;Reyes-Turcu FE;Zhang K;Rubin C;Grewal SI
兼性异染色质在细胞分化过程中发生变化，协调受调控的基因表达，但其组装尚不清楚。在这里，我们描述了兼性异染色质岛分裂酵母，并表明它们的形成在减数分裂基因需要的因素，消除减数分裂信使RNA（mRNA）在营养生长。阻断减数分裂mRNA的产生或RNA消除因子（包括Mmi 1和Red 1蛋白）的丢失，可消除异染色质岛。RNA消除机制在减数分裂位点富集，并与Clr 4/SUV 39 h相互作用，Clr 4/SUV 39 h是一种参与异染色质组装的甲基转移酶。异染色质岛解体的营养信号，诱导性别分化。这个过程涉及抗沉默因子Epe 1，其缺失导致异染色质基因座的急剧增加。我们的分析揭示了意想不到的mRNA加工因子组装动态异染色质调节基因表达的调节作用。
Facultative heterochromatin that changes during cellular differentiation coordinates regulated gene expression, but its assembly is poorly understood. Here, we describe facultative heterochromatin islands in fission yeast and show that their formation at meiotic genes requires factors that eliminate meiotic messenger RNAs (mRNAs) during vegetative growth. Blocking production of meiotic mRNA or loss of RNA elimination factors, including Mmi1 and Red1 proteins, abolishes heterochromatin islands. RNA elimination machinery is enriched at meiotic loci and interacts with Clr4/SUV39h, a methyltransferase involved in heterochromatin assembly. Heterochromatin islands disassemble in response to nutritional signals that induce sexual differentiation. This process involves the antisilencing factor Epe1, the loss of which causes dramatic increase in heterochromatic loci. Our analyses uncover unexpected regulatory roles for mRNA-processing factors that assemble dynamic heterochromatin to modulate gene expression.
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影响因子：
16.8
作者：
Zhang, Ke;Mosch, Kerstin;Grewal, Shiv I. S.
通讯作者：
Grewal, Shiv I. S.
影响因子：
4
作者：
Grewal, Shiv I. S.
通讯作者：
Grewal, Shiv I. S.
影响因子：
16.8
作者：
Woolcock, Katrina J.;Gaidatzis, Dimos;Buehler, Marc
通讯作者：
Buehler, Marc
DOI：
10.1073/pnas.0813063106
发表时间：
2009-06-02
影响因子：
11.1
作者：
Fischer, Tamas;Cui, Bowen;Grewal, Shiv I. S.
通讯作者：
Grewal, Shiv I. S.
影响因子：
16
作者：
Vasilieva, Lidia;Kim, Minkyu;Buratowski, Stephen
通讯作者：
Buratowski, Stephen