Properties of brush border vesicles isolated from rat kidney cortex by calcium precipitation.
Properties of brush border vesicles isolated from rat kidney cortex by calcium precipitation.
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通过钙沉淀从大鼠肾皮质分离的刷状缘囊泡的特性。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
R. Kinne
中科院分区：
文献类型：
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作者：
C. Evers;W. Haase;H. Murer;R. Kinne
在10 mM钙存在下，通过差速离心从大鼠肾皮质分离刷状缘膜囊泡。它们的特性进行了比较，刷状缘囊泡分离的自由流动电泳。通过钙沉淀法膜囊泡，在较短的时间内获得了类似的丰富的刷状缘标记酶（11- 12倍的碱性磷酸酶和麦芽糖酶），具有类似的降低活性的标记酶的基底侧质膜和一个几乎相同的蛋白质组合物所揭示的聚丙烯酰胺凝胶电泳在十二烷基硫酸钠。D-葡萄糖，L-苯丙氨酸和磷酸盐的两种膜制剂的运输性能基本相同;有一些迹象表明，由钙沉淀法制备的囊泡的钠渗透性较低。后者囊泡也显示出钠梯度刺激的L-谷氨酸盐摄取。
Brush border membrane vesicles were isolated from rat kidney cortex by differential centrifugation in the presence of 10 mM calcium. Their properties were compared to brush border vesicles isolated by free-flow electrophoresis. By the calcium precipitation method membrane vesicles were obtained in a shorter time with a similar enrichment of brush border marker enzymes (11- to 12-fold for alkaline phosphatase and maltase), with a similarly reduced activity of the marker enzyme for basal-lateral plasma membranes and an almost identical protein composition as revealed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The transport properties of the two membrane preparations for D-glucose, L-phenylalanine, and phosphate are essentially the same; there is some indication for a lower sodium permeability of the vesicles prepared by the calcium precipitation method. The latter vesicles were also shown to exhibit sodium gradient stimulated uptake of L-glutamate.