Effects of apatite cements on proliferation and differentiation of human osteoblasts in vitro
Effects of apatite cements on proliferation and differentiation of human osteoblasts in vitro
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DOI：
10.1016/j.biomaterials.2003.08.003
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发表时间：
2004-03-01
期刊：
影响因子：
14
通讯作者：
Nagayama, M
中科院分区：
文献类型：
--
作者：
Yuasa, T;Miyamoto, Y;Nagayama, M
虽然已知磷灰石水泥（AC）和烧结羟基磷灰石（s-HAP）显示出良好的骨传导性，但尚不清楚它们的骨传导性程度是否相同。此外，还没有澄清它是否取决于AC的类型;常规AC（c-AC）、快速凝固AC（fs-AC）或抗冲洗AC（aw-AC）。本研究的目的是研究AC对培养的人成骨细胞的影响，因为它们可能提供一个有用的骨传导性指标。将人成骨细胞在AC和s-HAP的表面上培养，并就细胞附着、增殖和分化进行评价。我们发现AC和s-HAP显示出相似的细胞附着。AC和s-HAP对成骨细胞的增殖无显著性差异。相比之下，我们发现与s-HAP相比，AC表面成骨细胞的分化增强。然而，AC类型之间没有差异。因此，我们得出结论，AC可能显示出比s-HAP更好的骨传导性，并且无论AC的类型如何，AC的骨传导性可能相似。(C)2003爱思唯尔有限公司。保留所有权利。
Although apatite cement (AC) and sintered hydroxyapatite (s-HAP) are known to show good osteoconductivity, it is not clear whether or not the degree of their osteoconductivity is the same. fit addition, it has not been clarified whether or not it is dependent on the type of AC; conventional AC (c-AC), fast-setting AC (fs-AC) or anti-washout AC (aw-AC). The aim of this study was to investigate the effects of ACs on cultured human osteoblasts, as they may provide a useful index of osteoconductivity. Human osteoblasts were cultured on the surfaces of ACs and s-HAP, and were evaluated with respect to cell attachment, proliferation, and differentiation. We found that ACs and s-HAP showed similar cell attachment. No significant difference between ACs and s-HAP was found with respect to the proliferation of osteoblasts. In contrast, we found that the differentiation of osteoblasts was enhanced on the surface of ACs compared with that of s-HAP. However, there was no difference among the types of AC. We therefore concluded that AC may show better osteoconductivity than s-HAP, and that osteoconductivity of AC may be similar, regardless of the type of AC. (C) 2003 Elsevier Ltd. All rights reserved.