Huntingtin aggregation monitored by dynamic light scattering
Huntingtin aggregation monitored by dynamic light scattering
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DOI：
10.1073/pnas.95.11.6118
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发表时间：
1998-05-26
影响因子：
11.1
通讯作者：
Wanker, EE
中科院分区：
文献类型：
--
作者：
Georgalis, Y;Starikov, EB;Wanker, EE
用动态光散射研究了谷胱甘肽S-转移酶-亨廷顿蛋白（GST-HD）融合蛋白溶液中纤维形成的初始阶段。已经检查了具有不同长度（20和51个残基）的聚-L-谷氨酰胺（polyGln）延伸的两个GST-HD系统。对于这两个系统，动力学的z-平均平移扩散系数（D-app）和它们的角度依赖性已经得到。我们的数据显示，在GST-HD 51和GST-HD20溶液中确实发生了聚集，但在前者中更为明显。因此，我们的方法提供了一个强有力的工具，定量测定GST-HD纤维在体外。
An initial stage of fibrillogenesis in solutions of glutathione S-transferase-huntingtin (GST-HD) fusion proteins has been studied by using dynamic light scattering. Two GST-HD systems with poly-L-glutamine (polyGln) extensions of different lengths (20 and 51 residues) have been examined. For both systems, kinetics of z-average translation diffusion coefficients (D-app) and their angular dependence have been obtained. Our data reveal that aggregation does occur in both GST-HD51 and GST-HD20 solutions, but that it is much more pronounced in the former. Thus, our approach provides a powerful tool for the quantitative assay of GST-HD fibrillogenesis in vitro.