Helicobacter pylori infection associates with fecal microbiota composition and diversity
Helicobacter pylori infection associates with fecal microbiota composition and diversity
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DOI：
10.1038/s41598-019-56631-4
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发表时间：
2019-12-27
影响因子：
4.6
通讯作者：
Lerch, Markus M.
中科院分区：
文献类型：
--
作者：
Frost, Fabian;Kacprowski, Tim;Lerch, Markus M.
幽门螺杆菌(H.)幽门螺杆菌是消化性溃疡最重要的病因，也是胃癌的危险因素。幽门螺杆菌的定植如何影响人类肠道微生物区系组成尚不清楚。我们在以人群为基础的波美拉尼亚健康研究(SHIP)趋势队列中调查了幽门螺杆菌感染与肠道微生物群组成的关系。反H。用幽门螺杆菌血清学和幽门螺杆菌粪便抗原试验确定幽门螺杆菌感染状况。采用16S rRNA基因测序方法，对212例Hp阳性和212例阴性对照的粪便微生物区系组成进行了分析。幽门螺杆菌感染与粪便微生物区系的改变和粪便微生物多样性的普遍增加显著相关。在受感染的个体中，幽门螺杆菌粪便抗原载量决定微生物变异的比例比年龄或性别更大。最高的幽门螺杆菌粪便抗原载量与可能有害的微生物群组成有关。这项研究表明，幽门螺杆菌感染者的粪便微生物区系发生了深刻的变化。虽然与幽门螺杆菌感染相关的微生物区系多样性的增加以及特定属丰度的变化可能被认为是有益的，但也可能与不利的健康影响有关，反映了幽门螺杆菌与其人类宿主之间的复杂关系。
Helicobacter (H.) pylori is the most important cause for peptic ulcer disease and a risk factor for gastric carcinoma. How colonization with H. pylori affects the intestinal microbiota composition in humans is unknown. We investigated the association of H. pylori infection with intestinal microbiota composition in the population-based cohort Study-of-Health-in-Pomerania (SHIP)-TREND. Anti-H. pylori serology and H. pylori stool antigen tests were used to determine the H. pylori infection status. The fecal microbiota composition of 212 H. pylori positive subjects and 212 matched negative control individuals was assessed using 16S rRNA gene sequencing. H. pylori infection was found to be significantly associated with fecal microbiota alterations and a general increase in fecal microbial diversity. In infected individuals, the H. pylori stool antigen load determined a larger portion of the microbial variation than age or sex. The highest H. pylori stool antigen loads were associated with a putatively harmful microbiota composition. This study demonstrates profound alterations in human fecal microbiota of H. pylori infected individuals. While the increased microbiota diversity associated with H. pylori infection as well as changes in abundance of specific genera could be considered to be beneficial, others may be associated with adverse health effects, reflecting the complex relationship between H. pylori and its human host.