Establishing pediatric reference intervals for 13 biochemical analytes derived from normal subjects in a pediatric endocrinology clinic in Korea
Establishing pediatric reference intervals for 13 biochemical analytes derived from normal subjects in a pediatric endocrinology clinic in Korea
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DOI：
10.1016/j.clinbiochem.2014.09.010
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发表时间：
2014-12-01
影响因子：
2.8
通讯作者：
Kim, Jeong-Ho
中科院分区：
文献类型：
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作者：
Cho, Sun-Mi;Lee, Sang-Guk;Kim, Jeong-Ho
目的：确定儿科参考间隔是实验室医生最困难的任务之一。成长中的孩子的生理学不断变化，使得他们的实验室价值不断移动目标。此外，种族和行为差异也可能导致差异。设计与方法：研究对象为2010年9月至2012年6月在韩国首尔塞维兰斯医院儿科内分泌科门诊就诊的2474例儿童，年龄2~14岁，男孩2~16岁，体格检查正常。用日立7600全自动生化分析仪检测血清钙、无机磷、尿素氮、肌酐、尿酸、血糖、总胆固醇、总蛋白、白蛋白、碱性磷酸酶、天冬氨酸氨基转移酶、丙氨酸氨基转移酶和总胆红素。参考区间按性别或年龄分组，采用Harris和Boyd方法。结果：除钙和白蛋白外，大多数分析物需要按性别或年龄进行分配。在按性别划分后，为男性和女性建立了碱性磷酸酶、肌酐和总胆红素的年龄特定分区参考区间。还需要对女性的天冬氨酸氨基转移酶和男性的总蛋白和尿酸进行年龄特定的额外分配。无机磷、总胆固醇、丙氨酸氨基转移酶、尿素氮和葡萄糖仅按性别划分。结论：利用现代分析化学平台，本研究为足够数量的健康儿童的13种基本血清化学分析提供了最新的年龄和性别特定的儿科参考区间。(C)2014年加拿大临床化学家学会。爱思唯尔公司出版，版权所有。
Objectives: Defining pediatric reference intervals is one of the most difficult tasks for laboratory physicians. The continuously changing physiology of growing children makes their laboratory values moving targets. In addition, ethnic and behavioral differences might also cause variations. The aim of this study was to establish age- and sex-specific partitioned reference intervals for 13 serum biochemical analytes in Korean children.Design and methods: A total of 2474 patients, girls aged 2-14 years and boys aged 2-16 years, who underwent a short stature workup but were diagnosed as normal at the Pediatric Endocrinology Clinic of Severance Hospital (Seoul, Korea) between September 2010 and June 2012 were included in this study. The levels of serum calcium, inorganic phosphorus, blood urea nitrogen, creatinine, uric acid, glucose, total cholesterol, total protein, albumin, alkaline phosphatase, aspartic aminotransferase, alanine aminotransferase, and total bilirubin were measured using a Hitachi 7600 analyzer (Hitachi High-Technologies Corporation, Tokyo, Japan). Reference intervals were partitioned according to sex or age subgroups using the Harris and Boyd method.Results: Most analytes except calcium and albumin required partitioning either by sex or age. Age-specific partitioned reference intervals for alkaline phosphatase, creatinine, and total bilirubin were established for both males and females after being partitioned by sex. Additional age-specific partitioning of aspartic amino-transferase in females and total protein and uric acid in males was also required. Inorganic phosphorus, total cholesterol, alanine aminotransferase, blood urea nitrogen, and glucose were partitioned only by sex.Conclusions: This study provided updated age- and sex-specific pediatric reference intervals for 13 basic serum chemistry analytes from a sufficient number of healthy children by using a modern analytical chemistry platform. (C) 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.