The uvrD gene of E. coli encodes a DNA-dependent ATPase
The uvrD gene of E. coli encodes a DNA-dependent ATPase
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大肠杆菌的 uvrD 基因编码 DNA 依赖性 ATP 酶
DOI：
10.1038/298098a0
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发表时间：
1982
期刊：
影响因子：
64.8
通讯作者：
M. Sekiguchi
中科院分区：
文献类型：
--
作者：
K. Oeda;T. Horiuchi;M. Sekiguchi
大肠杆菌 K-12 的 uvrD 基因突变会导致多种表型，包括对紫外线和电离辐射的敏感性增加、宿主细胞重新激活的能力降低、自发突变频率增加和基因重组率降低1-6。此外，已经表明，uvrD 和 polA 基因均发生突变的双突变体具有条件致死性7-10。 uvrD突变体的多效性表型表明uvrD基因产物可能在DNA修复、重组和复制过程中发挥重要作用。为了研究 uvrD 基因的功能，我们构建了携带该基因的杂交噬菌体和质粒11。在这里，我们分析了杂交噬菌体合成的蛋白质，并报告uvrD基因产物是分子量（MW）75,000的多肽。我们提供的证据表明 uvrD 蛋白具有 DNA 依赖性 ATP 酶活性。
Mutations in the uvrD gene of Escherichia coli K-12 result in multiple phenotypes, including increased sensitivity to UV light and ionizing radiation, decreased ability for host cell reactivation, increased spontaneous mutation frequency and decreased rate of genetic recombination1–6. It has been shown, moreover, that double mutants having mutations in both uvrD and polA genes are conditionally lethal7–10. The pleiotropic phenotypes of the uvrD mutants suggest that the uvrD gene product may have important roles in the processes of DNA repair, recombination and replication. To study the function of the uvrD gene, we have constructed hybrid phages and plasmids that carry the gene11. Here we have analysed the proteins synthesized by the hybrid phages and report that the uvrD gene product is a polypeptide of molecular weight (MW) 75,000. We present evidence that the uvrD protein possesses a DNA-dependent ATPase activity.