Transcriptomic insight into pathogenicity-associated factors of Conidiobolus obscurus, an obligate aphid-pathogenic fungus belonging to Entomopthoromycota
Transcriptomic insight into pathogenicity-associated factors of Conidiobolus obscurus, an obligate aphid-pathogenic fungus belonging to Entomopthoromycota
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转录组学对暗色耳霉（Conidiobolus obscurus）致病性相关因素的深入研究，暗色耳霉是一种属于昆虫真菌门的专性蚜虫致病真菌
DOI：
10.1002/ps.4861
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发表时间：
2018
影响因子：
4.1
通讯作者：
Montalva Cristian
中科院分区：
文献类型：
--
作者：
Wang Jianghong;Zhou Xiang;Guo Kai;Zhang Xinqi;Lin Haiping;Montalva Cristian
背景暗孢霉是一种分布广泛的昆虫病原真菌，具有生物防治蚜虫的潜力。本研究对C.首次从组装的17 231个真菌单基因中注释了致病性相关因子的数量，包括编码枯草杆菌蛋白酶样蛋白水解酶（Pr 1）、胰蛋白酶样蛋白酶、金属蛋白酶、羧肽酶和内切几丁质酶的那些。与体外真菌培养相比，这些基因中的许多在真菌化尸体中转录上调至少两倍。通过在真实的实时定量聚合酶链反应（PCR）中从不同的活体和体外材料中定量的三种选定的致病性相关基因的转录水平来验证所得的转录组数据库。还建议在真菌感染的第一阶段参与多种Pr 1蛋白酶。有趣的是，一个独特的溶细胞（Cyt）样δ-内毒素基因在真菌化的尸体和真菌培养物中高度表达，并或多或少不同于其在细菌和其他fungi.CONCLUSIONOUR的研究结果提供了第一个全球性的洞察到各种致病性相关基因在这种专性蚜虫病原体，并可能有助于开发新的生物防治策略对蚜虫害虫。© 2018化学工业协会
BACKGROUNDConidiobolus obscurusis a widespread fungal entomopathogen with aphid biocontrol potential. This study focused on ade novotranscriptomic analysis ofC. obscurus.RESULTSA number of pathogenicity‐associated factors were annotated for the first time from the assembled 17 231 fungal unigenes, including those encoding subtilisin‐like proteolytic enzymes (Pr1s), trypsin‐like proteases, metalloproteases, carboxypeptidases and endochitinases. Many of these genes were transcriptionally up‐regulated by at least twofold in mycotized cadavers compared with thein vitrofungal cultures. The resultant transcriptomic database was validated by the transcript levels of three selected pathogenicity‐related genes quantified from differentin vivoandin vitromaterial in real‐time quantitative polymerase chain reaction (PCR). The involvement of multiple Pr1 proteases in the first stage of fungal infection was also suggested. Interestingly, a unique cytolytic (Cyt)‐like δ‐endotoxin gene was highly expressed in both mycotized cadavers and fungal cultures, and was more or less distinct from its homologues in bacteria and other fungi.CONCLUSIONOur findings provide the first global insight into various pathogenicity‐related genes in this obligate aphid pathogen and may help to develop novel biocontrol strategy against aphid pests. © 2018 Society of Chemical Industry