Mycobacterium arosiense sp nov., a slowly growing, scotochromogenic species causing osteomyelitis in an immunocompromised child
Mycobacterium arosiense sp nov., a slowly growing, scotochromogenic species causing osteomyelitis in an immunocompromised child
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DOI：
10.1099/ijs.0.65503-0
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发表时间：
2008-10-01
影响因子：
2.8
通讯作者：
Thomsen, Vibeke Ostergaard
中科院分区：
文献类型：
--
作者：
Bang, Didi;Herlin, Troels;Thomsen, Vibeke Ostergaard
一种黄色、暗显色、生长缓慢的分枝杆菌菌株，命名为T1921（T），从一名7岁儿童的播散性骨髓炎病变中分离出，该儿童有潜在的部分γ干扰素受体α 1缺乏症。通过线性探针测定的杂交表明存在分枝杆菌属。16 S rRNA基因、ITS区、hsp 65和rpoB基因的序列分析表明，T1921（T）菌株可与所有已知的分枝杆菌属种区分开来。基于16 S rRNA基因的系统发育分析表明，菌株T1921（T）与胞内分枝杆菌（Mycobacterium intracellulare）的亲缘关系最近，而基于ITS、hsp 65和rpoB基因的系统发育分析表明，菌株T1921（T）与鸟分枝杆菌（Mycobacterium avium）的亲缘关系最近。表型试验不能区分菌株T1921（T）与类似的缓慢生长的分枝杆菌。菌株T1921（T）被认为是分枝杆菌属的一个新种，建议将其命名为分枝杆菌arosiense sp. nov.。模式菌株为T1921（T）（=DSM 45069（T）=ATCC BAA-1401（T））。
A yellow-pigmented, scotochromogenic, slowly growing mycobacterial strain, designated T1921(T), was isolated from the disseminated osteomyelitic lesions of a 7-year-old child with an underlying partial gamma interferon receptor alpha-1 deficiency. Hybridization by the line probe assay indicated the presence of a Mycobacterium species. Sequencing of the 16S rRNA gene, the internally transcribed spacer (ITS) region and the hsp65 and rpoB genes revealed that strain T1921(T) could be differentiated from all recognized species of the genus Mycobacterium. Phylogenetic analysis based on the 16S rRNA gene indicated that strain T1921(T) was related most closely to Mycobacterium intracellulare, whereas analysis based on the ITS and hsp65 and rpoB genes indicated that it was most closely related to Mycobacterium avium. Phenotypic tests were not able to differentiate strain T1921(T) from similar slowly growing mycobacteria. Strain T1921(T) is considered to represent a novel species of the genus Mycobacterium, for which the name Mycobacterium arosiense sp. nov. is proposed. The type strain is T1921(T) (=DSM 45069(T) =ATCC BAA-1401(T)).