Determination of carotenoids and their esters in fruits of Lycium barbarum Linnaeus by HPLC-DAD-APCI-MS
Determination of carotenoids and their esters in fruits of Lycium barbarum Linnaeus by HPLC-DAD-APCI-MS
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DOI：
10.1016/j.jpba.2008.04.001
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发表时间：
2008-08-05
影响因子：
3.4
通讯作者：
Chen, B. H.
中科院分区：
文献类型：
--
作者：
Inbaraj, B. Stephen;Lu, H.;Chen, B. H.
枸杞子是一种含有类胡萝卜素、黄酮类化合物和多糖等功能性成分的传统中草药，由于其可能在预防老年性黄斑变性等慢性疾病中发挥作用，已被广泛应用于保健食品行业。建立了大气压化学电离（APCl）模式下高效液相色谱-二极管阵列检测-质谱（HPLC-DAD-MS）联用技术，用于枸杞果实中类胡萝卜素的定性和定量分析。枸杞L.对枸杞进行提取而不皂化或提取后皂化。采用C30柱，以二氯甲烷（100%）-甲醇-乙腈-水（81：14：5，v/v/v）为移动的梯度流动相，从未皂化和皂化的L.枸杞子提取物分别在51和41 min内达到最佳提取效果。通过气相色谱法确定了类胡萝卜素酯的脂肪酸组成。玉米黄质二棕榈酸酯（1143.7 μ g/g）以最大量存在，其次是β-隐黄质棕榈酸酯及其两种异构体（32.9-68.5 μ g/g）、玉米黄质棕榈酸酯及其两种异构体（11.3-62.8 μ g/g）、全反式-β-胡萝卜素（23.7 μ g/g）和全反式-玉米黄质（1.4 μ g/g）。(C)2008 Elsevier B. V.保留所有权利。
The fruit of Lycium barbarum Linnaeus, a traditional Chinese herb containing functional components such as carotenoids, flavonoids and polysaccharides, has been widely used in the health food industry because of its possible role in the prevention of chronic disease like age-related macular degeneration. The objectives of this study were to develop a high performance liquid chromatography-photo diode array detection-mass spectrometry (HPLC-DAD-MS) method with atmospheric pressure chemical ionization (APCl) mode for qualitative and quantitative analyses of carotenoids in fruits of L. barbarum. Dried samples of L. barbarum were subjected to extraction without saponification or extraction followed by saponification. A C30 column with a gradient mobile phase of methylene chloride (100%) and methanol-acetonitrile-water (81:14:5, v/v/v) was used to separate carotenoids, with a total of 11 free carotenoids and 7 carotenoid esters being resolved from unsaponified and saponified L. barbarum extracts within 51 and 41 min, respectively. The fatty acid composition of carotenoid esters was confirmed by gas chromatography. Zeaxanthin dipalmitate (1143.7 mu g/g) was present in the largest amount, followed by beta-cryptoxanthin monopalmitate and its two isomers (32.9-68.5 mu g/g), zeaxanthin monopalmitate and its two isomers (11.3-62.8 mu g/g), all-trans-beta-carotene (23.7 mu g/g) and all-trans-zeaxanthin (1.4 mu g/g). (C) 2008 Elsevier B.V. All rights reserved.