Size effect of polydiacetylene vesicles functionalized with glycolipids on their colorimetric detection ability
Size effect of polydiacetylene vesicles functionalized with glycolipids on their colorimetric detection ability
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DOI：
10.1021/jp052580y
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发表时间：
2005-10-13
影响因子：
3.3
通讯作者：
Jiang, L
中科院分区：
文献类型：
--
作者：
Guo, CX;Boullanger, P;Jiang, L
本文研究了糖脂功能化聚二乙炔微囊的尺寸效应对其比色检测能力的影响。聚二乙炔囊泡中含有糖脂，可作为无色变色性质的模型体系。由于ConA与糖基结合，肉眼可以观察到从蓝色到红色的可见颜色变化，并通过可见吸收光谱进行定量检测。在实验中，通过不同孔径的膜挤压后，可以得到细小而均匀的囊泡。用透射电子显微镜(TEM)和动态光散射(DLS)分别研究了挤出囊泡的形态和平均尺寸分布。我们的工作表明，小泡越小，作用越强，从而使ConA的检测变得更容易。研究结果可用于提高聚二乙炔生物传感器对其他生物分子的识别灵敏度。
In this paper, the size effect of the polydiacetylene vesicles functionalized with glycolipids on their colorimetric detection ability has been studied. Polydiacetylene vesicles, in which were incorporated glycolipids acted as a model system for the affinochromatic property. Visible color changes from blue to red could be observed to the naked eye owing to Con A binding to the sugar moiety and be detected quantitatively by the visible absorption spectrum. In the experiment, small and uniform vesicles were obtained after extrusion through membranes with different pore sizes. The morphology and mean size distribution of the extruded vesicles were studied by transmission electron microscopy (TEM) and dynamic light scattering (DLS), respectively. Our work shows that the smaller the vesicles are, the stronger is the effect, making the detection of Con A easier. The results may apply to the sensitivity enhancement of polydiacetylene biosensors for the recognition of other biomolecules.