Effects of glaucoma medications on corneal endothelium, keratocytes, and subbasal nerves among participants in the ocular hypertension treatment study
Effects of glaucoma medications on corneal endothelium, keratocytes, and subbasal nerves among participants in the ocular hypertension treatment study
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DOI：
10.1097/01.ico.0000230499.07273.c5
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发表时间：
2006-10-01
期刊：
影响因子：
2.8
通讯作者：
Bourne, William M.
中科院分区：
文献类型：
--
作者：
Baratz, Keith H.;Nau, Cherie B.;Bourne, William M.
目的：比较角膜基底下神经和角膜细胞密度和内皮特征的高眼压患者治疗药物或observation.Methods：在高眼压治疗研究（OHTS）的参与者随机在马约诊所的药物或观察进行了评估，每年与角膜内皮显微镜6年。共聚焦显微镜检查在入组后78至108个月进行。通过手工描记和数字图像分析计算基底下神经密度。采用手工计数法测定角膜细胞密度。结果：6年后，观察组（n = 21）和药物组（n = 26）角膜内皮细胞密度、六角形细胞百分比和细胞面积变异系数相似（分别为2415 +/- 300 vs. 2331 +/- 239 cells/mm（2）; 63% +/- 11% vs. 65% +/- 10%;和0.32 +/- 0.07 vs. 0.30 +/- 0.06）。在38名接受共焦检查的参与者中，药物组（n = 19）的神经较少（3.8 ± 2.1 vs. 5.9 ± 2.0神经/帧; P = 0.02）和较低的神经密度（5643 +/- 2861 vs. 9314 +/- 3743 mu m/mm（2）; P = 0.007）比观察组患者（n = 10）。观察组中的另外9名患者在共聚焦扫描前开始用药，具有中等神经密度。全层角膜细胞密度相似，观察组和药物组分别为22，257 +/- 2419和23，430 +/- 3285 cell/mm（3）。结论：长期使用青光眼药物可导致角膜基底下神经纤维束的数量和密度减少，但不影响角膜细胞密度或角膜内皮特性。
Purpose: To compare subbasal corneal nerve and keratocyte density and endothelial characteristics of ocular hypertensive patients treated with medications or observation.Methods: Participants in the Ocular Hypertensive Treatment Study (OHTS) randomized at Mayo Clinic to medication or observation were evaluated with specular microscopy annually for 6 years. Confocal microscopy was performed 78 to 108 months after enrollment. Subbasal nerve density was calculated by manual tracing and digital image analysis. Keratocyte density was determined by manual counting methods. Data were compared using a t test and a rank sum test.Results: After 6 years, corneal endothelial cell density, percent hexagonal cells, and coefficient of variation of cell area for the observation (n = 21) and medication groups (n = 26) were similar (2415 +/- 300 vs. 2331 +/- 239 cells/mm(2); 63% +/- 11% vs. 65% +/- 10%; and 0.32 +/- 0.07 vs. 0.30 +/- 0.06, respectively). Of 38 participants undergoing confocal examination, the medication group (n = 19) had fewer nerves (3.8 +/- 2.1 vs. 5.9 +/- 2.0 nerves/frame; P = 0.02) and a lower nerve density (5643 +/- 2861 vs. 9314 +/- 3743 mu m/mm(2); P = 0.007) than the observation patients (n = 10). An additional 9 patients in the observation group, who began medication before confocal scanning, had intermediate nerve densities. Full-thickness keratocyte density was similar, with 22,257 +/- 2419 and 23,430 +/- 3285 cell/mm(3) in the observation and medication groups, respectively.Conclusions: Chronic administration of glaucoma medications causes a decrease in the number and density of corneal subbasal nerve fiber bundles but does not affect keratocyte density or corneal endothelial characteristics.