Evaluation of a Recombinant Multiepitope Peptide for Serodiagnosis of Toxoplasma gondii Infection
Evaluation of a Recombinant Multiepitope Peptide for Serodiagnosis of Toxoplasma gondii Infection
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DOI：
10.1128/cvi.05553-11
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发表时间：
2012-03-01
影响因子：
--
通讯作者：
Si, Jin
中科院分区：
文献类型：
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作者：
Dai, Jianfang;Jiang, Min;Si, Jin
用敏感、特异的方法检测弓形虫感染是弓形虫病防治的关键步骤。在可用的诊断测试中，血清学是常用的。虽然血清学试验给出了令人满意的结果，但可靠试剂的生产仍然费力且昂贵。因此，真实的需要获得特异性和有效的重组抗原用于T.弓形虫感染在这项研究中，多表位肽的设计，并成功地在大肠杆菌中表达，然后IgG和IgM酶联免疫吸附试验（ELISA）的发展和评估。结果表明，该多表位抗原是一种很有前途的重组抗原，可用于弓形虫病的常规筛查。
Detection of Toxoplasma gondii infection with sensitive and specific methods is a key step in the prevention and treatment of toxoplasmosis. Among the available diagnostic tests, serology is commonly used. Although serological tests give satisfactory results, the production of reliable reagents remains laborious and expensive. There is therefore a real need to acquire specific and effective recombinant antigens for the serodiagnosis of T. gondii infection. In this study, a multiepitope peptide was designed and successfully expressed in Escherichia coli, and then IgG and IgM enzyme-linked immunosorbent assays (ELISAs) were developed and evaluated. Our results showed that the new multiepitope antigen is one of the most promising recombinant antigens which could be used in routine screening of human toxoplasmosis.