Effects of stretching stress on the muscle contraction proteins of skeletal muscle myoblasts
Effects of stretching stress on the muscle contraction proteins of skeletal muscle myoblasts
复制标题
DOI：
10.2220/biomedres.26.61
复制
发表时间：
2005-04-01
影响因子：
1.2
通讯作者：
Ide, Yoshinobu
中科院分区：
文献类型：
--
作者：
Sakiyama, Koji;Abe, Shinichi;Ide, Yoshinobu
一些研究报道，通过施加适当的机械刺激可以促进培养的成肌细胞的生长和分化。然而，机械刺激对肌纤维特性的影响尚未完全阐明。在这项研究中，我们对 C2C12 细胞施加机械应力，C2C12 细胞是源自小鼠骨骼肌的成肌细胞。为了阐明肌肉特征，研究了以下肌球蛋白重链 (MHC) 同工型：MHC-2b、2d 和 2a，它们都是快肌纤维。将细胞接种到硅胶室上后，对室进行机械拉伸，并使用LightCycler(TM)多次测量每种MHC亚型的mRNA表达。结果表明，通过机械拉伸，MHC-2b 的表达最初较高。另一方面，在没有拉伸的情况下，MHC-2d的表达随着时间的推移而增加，但在拉伸的情况下，几乎看不到这种情况。此外，拉伸组中MHC-2a的表达显着升高。这项研究的这些结果表明，当间歇性刺激时，成肌细胞表达的 MHC-2a 亚型水平会增加。因此，表明肌细胞对环境变化的反应不仅促进生长和分化，而且在MHC亚型水平上主动改变肌肉功能。
Several studies have reported that growth and differentiation of cultured myoblasts can be facilitated by applying appropriate mechanical stimulus. However, the effects of mechanical stimulus on the characteristics of muscle fibers have not yet been fully elucidated. In this study, we gave mechanical stress to C2C12 cells, which were myoblasts derived from mice skeletal muscle. The following myosin heavy chain (MHC) isoforms were investigated in order to clarify muscle characteristics: MHC-2b, 2d and 2a, all of which are fast-twitch fibers. After inoculating cells on a silicone chamber, the chamber was mechanically stretched, and a LightCycler (TM) was used to measure the mRNA expression of each MHC isoform at several times. The results showed that, with mechanical stretching, the expression of MHC-2b was initially high. On the other hand, without stretching, the expression of MHC-2d increased over time, but with stretching, it was hardly seen. Furthermore, the expression of MHC-2a was significantly high in the stretching group. These results of this study suggest that, when intermittently stimulated, myoblasts express increased levels of MHC-2a isoform. Therefore, it is indicated that myocytes respond to environmental changes not only to facilitate growth and differentiation, but also to alter muscle function actively at the MHC isoform level.