STABILITY OF NUCLEAR-RNA IN MAMMALIAN-CELLS
STABILITY OF NUCLEAR-RNA IN MAMMALIAN-CELLS
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DOI：
10.1016/0022-2836(74)90444-6
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发表时间：
1974-01-01
影响因子：
5.6
通讯作者：
MCCONKEY, EH
中科院分区：
文献类型：
--
作者：
BRANDHORST, BP;MCCONKEY, EH
测定和分析了[~3H]腺苷进入小鼠L细胞三磷酸腺苷、细胞核糖核酸和核糖核酸的动力学。根据核糖核酸和三磷酸腺苷的比放射性估算了核糖核酸的摩尔累积曲线；该曲线被图解为稳定组分和不稳定组分。应用一阶衰变分析对不稳定组分(主要是异质核RNA)的稳定性进行了估计。异质的核核糖核酸衰变的半衰期明显一致，为23分钟，比之前的一些估计要大得多。它的瞬时合成速率为5.4x10−2pg/min，在细胞核内达到1.8pg/细胞的稳态水平，占细胞总核糖核酸的7%。在L细胞合成的异源核糖核酸中，只有约2%以信使核糖核酸的形式进入多聚体。参考海胆胚胎的类似确定值，讨论了这些值的含义。
The kinetics of entry of [3H]adenosine into ATP, cellular RNA, and nuclear RNA of mouse L cells were determined and analyzed. A molar accumulation curve for RNA was estimated from the specific radioactivities of RNA and ATP; this curve was resolved graphically into stable and unstable components. The stability of the unstable component (mostly heterogeneous nuclear RNA) was estimated by applying first-order decay analysis. Heterogeneous, nuclear RNA decays with an apparently uniform half-life of 23 minutes, considerably greater than some previous estimates. It is synthesized at an instantaneous rate of 5.4 × 10−2pg/cell per minute and reaches a steady-state level of 1.8 pg/cell in the nucleus, or 7% of the total cellular RNA. Only about 2% of the heterogeneous RNA synthesized in L cells enters polysomes as messenger RNA. The implications of these values are discussed with reference to similarly determined values for sea urchin embryos.