Persistent Organic Pollutant Burden, Experimental POP Exposure, and Tissue Properties Affect Metabolic Profiles of Blubber from Gray Seal Pups.
Persistent Organic Pollutant Burden, Experimental POP Exposure, and Tissue Properties Affect Metabolic Profiles of Blubber from Gray Seal Pups.
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DOI：
10.1021/acs.est.8b04240
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发表时间：
2018-10
影响因子：
11.4
通讯作者：
Kelly J. Robinson;Ailsa J Hall;C. Debier;G. Eppe;J. Thomé;Kimberley A Bennett
中科院分区：
文献类型：
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作者：
Kelly J. Robinson;Ailsa J Hall;C. Debier;G. Eppe;J. Thomé;Kimberley A Bennett
持久性有机污染物（POPs）是有毒的，无处不在，抗分解，在活组织中生物积累，并在食物网中生物放大。持久性有机污染物还会改变人类和野生动物体内的能量平衡。海洋哺乳动物经历高POP浓度，但对其组织代谢特性的影响尚不清楚。我们使用野生灰海豹幼崽的脂肪外植体来研究内在组织POP负担和急性实验性POP暴露对脂肪代谢特性的影响。葡萄糖的使用、乳酸的产生和脂溶率在来自同一个体的匹配的内外脂肪外植体之间以及在喂食和自然禁食之间存在差异。葡萄糖的使用随脂肪二恶英样多氯联苯（DL-PCB）而减少，随急性实验性POP暴露而增加。乳酸产量在饲养期间随着DL-PCBs的增加而增加，但在禁食期间随着DL-PCBs的增加而减少。脂肪分解速率随脂肪脂二氯二苯三氯乙烷及其代谢物（DDX）的添加而增加，随DDX的添加而降低。我们的数据显示，海豹幼崽体内的POP负担足够高，足以改变它们生命早期的脂肪功能，此时脂肪沉积和动员至关重要。这种pop诱导的脂肪代谢特性的改变可能会显著改变海洋顶级捕食者的能量平衡调节，并可能对健康和生存产生长期影响。
Persistent organic pollutants (POPs) are toxic, ubiquitous, resist breakdown, bioaccumulate in living tissue, and biomagnify in food webs. POPs can also alter energy balance in humans and wildlife. Marine mammals experience high POP concentrations, but consequences for their tissue metabolic characteristics are unknown. We used blubber explants from wild, gray seal ( Halichoerus grypus) pups to examine impacts of intrinsic tissue POP burden and acute experimental POP exposure on adipose metabolic characteristics. Glucose use, lactate production, and lipolytic rate differed between matched inner and outer blubber explants from the same individuals and between feeding and natural fasting. Glucose use decreased with blubber dioxin-like PCBs (DL-PCB) and increased with acute experimental POP exposure. Lactate production increased with DL-PCBs during feeding, but decreased with DL-PCBs during fasting. Lipolytic rate increased with blubber dichlorodiphenyltrichloroethane and its metabolites (DDX) in fasting animals, but declined with DDX when animals were feeding. Our data show that POP burdens are high enough in seal pups to alter adipose function early in life, when fat deposition and mobilization are vital. Such POP-induced alterations to adipose metabolic properties may significantly alter energy balance regulation in marine top predators, with the potential for long-term impacts on fitness and survival.