Evaluation of a simple off-the-shelf bi-layered vascular scaffold based on poly(L-lactide-co-ε-caprolactone)/silk fibroin in vitro and in vivo
Evaluation of a simple off-the-shelf bi-layered vascular scaffold based on poly(L-lactide-co-ε-caprolactone)/silk fibroin in vitro and in vivo
复制标题
基于聚（L-丙交酯-共-ε-己内酯）/丝素蛋白的简单现成双层血管支架的体外和体内评估
DOI：
10.2147/ijn.s205569
复制
发表时间：
2019-01-01
影响因子：
8
通讯作者：
Yin, Meng
中科院分区：
文献类型：
--
作者：
Jin, Dawei;Hu, Junfeng;Yin, Meng
目的：在小口径血管支架研究领域，良好的血管重构是保证其抗凝功能的关键。材料与方法：以聚（L-丙交酯-co-E-己内酯）（PLCL）、丝素蛋白（SF）和肝素（Hep）为原料，采用静电纺丝法制备PLCL/SF/Hep双层支架和PLCL/Hep双层支架。内层为PLCL/SF/Hep或PLCL/Hep复合膜，外层为PLCL/SF纳米纱线。体外试验包括亲水性试验、机械性能试验以及血液和细胞相容性评价。结果：PLCL/SF/Hep-2膜的亲水性较PLCL/Hep-2膜有明显改善，且与PLCL/SF/Hep-2膜相比，PLCL/SF/Hep-2膜的亲水性有明显提高。机械强度满足使用要求。血液和细胞相容性都是最佳的。最重要的是，PLCL/SF/Hep支架在活体兔颈动脉移植后3个月内保持管腔通畅。结论：PLCL/SF/Hep复合支架具有良好的血管重构效果。
Purpose: In the field of small-caliber vascular scaffold research, excellent vascular remodeling is the key to ensuring anticoagulant function. We prepared an off-the-shelf bi-layered vascular scaffold with a dense inner layer and a loose outer layer and evaluated its remodeling capabilities by in vivo transplantation.Materials and Methods: Based on poly(L-lactide-co-E-caprolactone) (PLCL), silk fibroin(SF), and heparin (Hep), PLCL/SF/Hep bi-layered scaffolds and PLCL/Hep bi-layered scaffolds were prepared by electrospinning. The inner layer was a PLCL/SF/Hep or PLCL/Hep nanofiber membrane, and the outer layer was PLCL/SF nano yarn. The in vitro tests included a hydrophilicity test, mechanical properties test, and blood and cell compatibility evaluation. The in vivo evaluation was conducted via single rabbit carotid artery replacement and subsequent examinations, including ultrasound imaging, immunoglobulin assays, and tissue section staining.Results: Compared to the PLCL/Hep nanofiber membrane, the hydrophilicity of the PLCL/SF/Hep nanofiber membrane was significantly improved. The mechanical strength met application requirements. Both the blood and cell compatibility were optimal. Most importantly, the PLCL/SF/Hep scaffolds maintained lumen patency for 3 months after carotid artery transplantation in live rabbits. At the same time, CD31 and a-SMA immunofluorescence staining confirmed bionic endothelial and smooth muscle layers remodeling.Conclusion: Using this hybrid strategy, PLCL and SF were combined to manufacture bi-layered small-caliber vascular scaffolds; these PLCL/SF/Hep scaffolds showed satisfactory vascular remodeling.