Defective Pollen Wall 3 (DPW3), a novel alpha integrin-like protein, is required for pollen wall formation in rice
Defective Pollen Wall 3 (DPW3), a novel alpha integrin-like protein, is required for pollen wall formation in rice
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缺陷花粉壁 3 (DPW3) 是一种新型 α 整合素样蛋白，是水稻花粉壁形成所必需的
DOI：
10.1111/nph.16161
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发表时间：
2020-01-01
期刊：
影响因子：
9.4
通讯作者：
Zhang, Dabing
中科院分区：
文献类型：
--
作者：
Mondol, Palash Chandra;Xu, Dawei;Zhang, Dabing
在开花植物中，花粉壁是包围雄性配子体的一种特殊的胞外细胞壁基质，是花粉粒抵御各种环境和生物胁迫的天然保护器。花粉壁的形成是一个复杂而调控良好的过程，涉及多种不同基因的作用。然而，这一过程背后的遗传和分子机制在很大程度上仍然未知。在本研究中，我们分离并鉴定了一个新的水稻雄性不育突变体——缺陷花粉壁3 (dpw3)，该突变体的花药较小，颜色较浅，花粉粒流产。DPW3编码一种在陆地植物中保守的新型膜相关α整合素样蛋白。DPW3在花药发育阶段普遍表达，其蛋白定位于质膜、内质网和高尔基体。dpw3植物的花药表现为花药角质层轮廓不平衡、乌氏体异常、胼胝质沉积紊乱、花粉壁形成缺陷（如小孢子质膜波动异常、原胺形成缺陷），导致花粉败育和雄性完全不育。我们的研究结果揭示了α整合素样蛋白在植物雄性生殖中的重要作用。
In flowering plants, pollen wall is a specialized extracellular cell-wall matrix surrounding male gametophytes and acts as a natural protector of pollen grains against various environmental and biological stresses. The formation of pollen wall is a complex but well-regulated process, which involves the action of many different genes. However, the genetic and molecular mechanisms underlying this process remain largely unknown.In this study, we isolated and characterized a novel rice male sterile mutant, defective pollen wall3 (dpw3), which displays smaller and paler anthers with aborted pollen grains. DPW3 encodes a novel membrane-associated alpha integrin-like protein conserved in land plants. DPW3 is ubiquitously expressed in anther developmental stages and its protein is localized to the plasma membrane, endoplasmic reticulum (ER) and Golgi.Anthers of dpw3 plants exhibited unbalanced anther cuticular profile, abnormal Ubisch bodies, disrupted callose deposition, defective pollen wall formation such as abnormal microspore plasma membrane undulation and defective primexine formation, resulting in pollen abortion and complete male sterility.Our findings revealed a novel and vital role of alpha integrin-like proteins in plant male reproduction.