Nanopore formation process in artificial cell membrane induced by plasma-generated reactive oxygen species
Nanopore formation process in artificial cell membrane induced by plasma-generated reactive oxygen species
复制标题
等离子体产生的活性氧诱导人工细胞膜纳米孔形成过程
DOI：
10.1016/j.abb.2016.05.014
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
M. Ito
中科院分区：
文献类型：
--
作者：
R. Tero;R. Yamashita;H. Hashizume;Y. Suda;H. Takikawa;M. Hori;M. Ito
我们研究了非平衡大气压等离子体产生的氧自由基诱导的人工脂质双层膜的形态变化。在不同的照射时间和距离条件下，对存在于缓冲溶液下的支撑脂质双层中的中性氧类O(3Pj)和O2(1Δg)进行辐照，定量计算氧类的剂量。使用原子力显微镜和荧光显微镜观察发现，一定剂量的中性氧在磷脂双层中产生直径为10-50 nm的纳米孔，并最终破坏双层结构。我们发现在纳米孔形成之前，脂质双层表面就出现了突起，我们将突起归因于纳米孔的前体。我们在之前的实验和理论研究的基础上提出了脂质氧化诱导孔隙形成的机制。
We investigated morphological change of an artificial lipid bilayer membrane induced by oxygen radicals which were generated by non-equilibrium atmospheric pressure plasma. Neutral oxygen species, O(3Pj) and O2(1Δg), were irradiated of a supported lipid bilayer existing under a buffer solution at various conditions of dose time and distances, at which the dose amounts of the oxygen species were calculated quantitatively. Observation using an atomic force microscope and a fluorescence microscope revealed that dose of the neutral oxygen species generated nanopores with the diameter of 10–50 nm in a phospholipid bilayer, and finally destructed the bilayer structure. We found that protrusions appeared on the lipid bilayer surface prior to the formation of nanopores, and we attributed the protrusions to the precursor of the nanopores. We propose a mechanism of the pore formation induced by lipid oxidation on the basis of previous experimental and theoretical studies.