RESOLUTION AND ASSIGNMENT OF THE 270-MHZ PROTON SPECTRUM OF CELLOBIOSE BY HOMO-NUCLEAR AND HETERONUCLEAR TWO-DIMENSIONAL NMR
RESOLUTION AND ASSIGNMENT OF THE 270-MHZ PROTON SPECTRUM OF CELLOBIOSE BY HOMO-NUCLEAR AND HETERONUCLEAR TWO-DIMENSIONAL NMR
复制标题
同核和异核二维核磁共振对纤维二糖270-MHZ质子谱的解析和归属
DOI：
10.1021/ja00525a057
复制
发表时间：
1980
影响因子：
15
通讯作者：
S. Sukumar
中科院分区：
文献类型：
--
作者：
L. Hall;G. Morris;S. Sukumar
(5)所用的细胞与参考文献3a中描述的细胞相似。中间相的外部面积（aq I）为3.43 cm 2，内部面积（aq II）为3.65 cm 2。(6)当将aq I调节至pH 8时，观察到诱导期（约50 h），在此期间氯仿相中的核苷酸浓度逐渐增加至稳态值（1.27 × 10-5 M）。表I中列出的转运速率是在稳态下获得的值。当在aq I中将pH设定为5.0时，未观察到明显的诱导期。(7)分析发现，运输的ADP在68 h后被水解为AMP。
(5) The cell used was similar tothe one described in ref 3a. The outer area of the interphase (aq I) was 3.43 cm2 and the inner (aq II) was 3.65 cm2.(6) When aq I was adjusted to pH 8, an induction period (~ 50 h) was observed during which the nucleotide concentration in the chloroform phase increased gradually to a steady-state value (1.27 X 10-5 M). The transport rates listed in Table I are values obtained at the steady state. When pH was set to 5.0 in aq I, no appreciable induction period was observed.(7) Analysis found that 85% of ADP transported was hydrolyzed to AMP after 68 h.