YEAST TATA-BINDING PROTEIN TFIID BINDS TO TATA ELEMENTS WITH BOTH CONSENSUS AND NONCONSENSUS DNA-SEQUENCES
YEAST TATA-BINDING PROTEIN TFIID BINDS TO TATA ELEMENTS WITH BOTH CONSENSUS AND NONCONSENSUS DNA-SEQUENCES
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DOI：
10.1073/pnas.86.15.5718
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发表时间：
1989-08-01
影响因子：
11.1
通讯作者：
GUARENTE, L
中科院分区：
文献类型：
--
作者：
HAHN, S;BURATOWSKI, S;GUARENTE, L
研究了酵母TATA元件结合蛋白TFIID的DNA结合特性。TFIID对腺病毒主要晚期启动子共识TATA元件的亲和力(表观平衡解离常数)为2倍。10-9M，这个值类似于基因特异性调节蛋白对其结合部位的亲和力。TFIID结合是高度特异的，可以识别亲和力低105倍的非特异位点。尽管有这种特异性，TFIID也与几个与一致的TATA序列(TATAAA和TATATA)不匹配的TATA元件具有高亲和力：酵母LEU2 TATA(TATTATTA)、猿猴病毒40 TATA(CTTATTTAT)和酵母Cyc1-10 TATA(TTATACATT)都与TFIID结合。此外，TFIID还能促进非共识TATA元件的体外转录。因此，与先前的建议相反，非共识塔塔因素的存在本身并不意味着存在多个塔塔约束因素。
The DNA binding properties of the yeast TATA element-binding protein TFIID were investigated. The affinity (apparent equilibrium dissociation constant) of TFIID for the adenovirus major late promoter consensus TATA element is 2 .times. 10-9 M, a value similar to the affinity of gene-specific regulatory proteins for their binding sites. TFIID binding is highly specific and recognizes nonspecific sites with .apprxeq.105-fold lower affinity. Despite this specificity, TFIID also binds with high affinity to several TATA elements that do not match the consensus TATA sequences (TATAAA and TATATA): the yeast LEU2 TATA (TATTATTTA), the simian virus 40 TATA (CTTATTTAT), and the yeast CYC1 -10 TATA (TTATACATT) all bound TFIID. Furthermore, TFIID was active in promoting transcription in vitro from the nonconsensus TATA elements. Thus, contrary to previous suggestions, the existence of nonconsensus TATA elements does not itself indicate the existence of multiple TATA-binding factors.