An in vitro bioassay for leukocytic endogenous mediator(s) using cultured rat hepatocytes.
An in vitro bioassay for leukocytic endogenous mediator(s) using cultured rat hepatocytes.
复制标题
使用培养的大鼠肝细胞对白细胞内源性介质进行体外生物测定。
DOI：
10.1007/bf00911093
复制
发表时间：
1981
期刊：
影响因子：
5.1
通讯作者：
Fuller,GM
中科院分区：
文献类型：
--
作者：
Ritchie,DG;Fuller,GM
Primary cultures of adult rat hepatocytes were used to assay for the presence of leukocytic endogenous mediator(s) (LEM), a neutrophil derived protein(s) capable of stimulating the synthesis of acute-phase plasma proteins when injected into rats. In the presence of physiological concentrations of dexamethasone (40 nM), the hepatocytes secreted a variety of plasma proteins as demonstrated by crossed immunoelectrophoresis. The addition of LEM to hepatocytes increased the secretion of several acute-phase related plasma proteins, including fibrinogen and hepatoglobin, and decreased albumin secretion. These results mimic the acute-phase response observed in the intact animal. Fibrinogen secretion was used as a quantitative marker for determining LEM activity. The rate of fibrinogen secretion depended upon both the concentration of dexamethasone and LEM present during a given 24-h assay period. One unit of LEM activity is defined as that concentration of LEM capable of producing a 50% maximal stimulation of fibrinogen secretion.
