AGE-CORRELATED CHANGES IN THE DNA-TEMPLATE IN THE NEMATODE CAENORHABDITIS-ELEGANS
AGE-CORRELATED CHANGES IN THE DNA-TEMPLATE IN THE NEMATODE CAENORHABDITIS-ELEGANS
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DOI：
10.1016/0047-6374(83)90080-5
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发表时间：
1983-01-01
影响因子：
5.3
通讯作者：
DECHAVIGNY, A
中科院分区：
文献类型：
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作者：
KLASS, M;NGUYEN, PN;DECHAVIGNY, A
从线虫C.线虫表现出许多与年龄相关的显著变化。单链断裂的数量测定的体外试验程序，使用大肠杆菌DNA聚合酶I显着增加，随着年龄的增长。在C.线虫的DNA随着蠕虫的成熟和衰老而变化。DNA，分离自老年蠕虫，表现出降低的转录能力时，在HeLa细胞体外转录系统进行测定。一项生物学试验，以确定年龄相关的变化，在老化的精子的DNA证明了显着减少的能力，精子支持合子发育的男性年龄增加。研究结果表明，显着的年龄相关的改变和修改发生在线虫的DNA模板，其影响的老化过程进行了讨论。
Analysis of DNA from the nematode C. elegans demonstrated a number of significant age-correlated changes. The number of single-strand breaks as assayed by an in-vitro assay procedure using Escherichia coli DNA polymerase I increased significantly with age. There was also an exponential increase in the amount of 5-methylcytosine in C. elegans DNA as the worm matured and aged. DNA, isolated from older worms, exhibited reduced transcriptional capacity when assayed in a HeLa cell in-vitro transcription system. A biological assay to determine age-correlated changes in the DNA of aging sperm demonstrated a significant reduction in the capacity of the sperm to support zygotic development as the age of the male increased. Findings demonstrated significant age-correlated alterations and modifications occurring in the DNA template of the nematode, and their implications to the aging process are discussed.