Alleviation of estrogen-induced hyperprolactinemia through intracerebral transplantation of hypothalamic tissue containing dopaminergic neurons.
Alleviation of estrogen-induced hyperprolactinemia through intracerebral transplantation of hypothalamic tissue containing dopaminergic neurons.
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通过脑内移植含有多巴胺能神经元的下丘脑组织来缓解雌激素引起的高催乳素血症。
DOI：
10.1159/000124551
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发表时间：
1986
影响因子：
4.1
通讯作者：
Leung,PC
中科院分区：
文献类型：
--
作者：
Arendash,GW;Leung,PC
通过长时间的雌激素治疗，可以在幼鼠中诱发分泌催乳素的垂体瘤。最近的证据表明，这种肿瘤与结节漏斗部多巴胺能（TI-DA）神经元的变性有关，TI-DA神经元通常抑制垂体前叶的催乳素分泌。在本研究中，在切除卵巢的Fischer 344大鼠中，通过皮下植入17β-雌二醇的硅橡胶胶囊1个月，诱导慢性高泌乳素血症。雌激素诱导的高催乳素血症大鼠接受新生儿延髓正中隆起（ME）组织（含TI-DA神经元）或杏仁核（对照）组织移植，置于第三脑室或下丘脑内。移植后1个月采集血样，用放射免疫法测定催乳素浓度。与对照组动物的平均值相比，接受心室放置的β-ME移植的4只动物中的2只和接受双侧β-ME移植的7只动物中的4只显示血浆催乳素水平大幅降低。后续的儿茶酚胺（CA）组织化学表明，在有效的EAE移植动物的正中隆起中有明亮的荧光强度;这与无效移植中保持高催乳素血症的动物的正中隆起内的弱CA荧光强度形成对比。此外，在形成鲜明对比的非常低的，非脉动LH水平在第二次出血的受体轴承无效的移植，受体与有效的MEATE-ME移植有高的，脉动水平的LH特性的正常，卵巢切除大鼠。这些数据表明，开发TI-DA神经元，有效的多巴胺-ME移植，成为功能恢复或加重DA抑制催乳素分泌，并在这样做，以及间接正常化LH分泌。
Prolactin-secreting pituitary tumors can be induced in young rats through prolonged estrogen treatment. Recent evidence suggests that such tumors are associated with a degeneration of tuberoinfundibular dopaminergic (TI-DA) neurons, which normally inhibit prolactin secretion by the anterior pituitary’s lactrotrophs. For this study, chronic hyperprolactinemia was induced in young, ovariectomized Fischer 344 rats through Silastic capsule implants of 17β-estradiol, placed subcutaneously for 1 month prior to removal. Rats with such estrogen-induced hyperprolactinemia then received transplants of neonatal arcuate-median eminence (ME) tissue (containing TI-DA neurons) or amygdala (control) tissue, placed either within the third ventricle or bilaterally within the hypothalamus. Blood samples were obtained 1 month after transplantation and prolactin concentrations measured by radioimmunoassay. Two of 4 animals receiving ventricularly-placed arcuate-ME transplants and 4 of 7 animals receiving bilateral arcuate-ME transplants showed substantial reductions in plasma prolactin levels compared to mean values in control animals. Follow-up catecholamine (CA) histochemistry indicated a bright fluorescence intensity in the median eminence of animals bearingeffectivearcuate-ME transplants; this, in contrast to the weak CA fluorescence intensity within the median eminence of animals remaining hyperprolactinemic withineffectivetransplants. Furthermore, in sharp contrast to the very low, nonpulsatile LH levels found during a second bleeding in recipients bearing ineffective transplants, recipients with effective arcuate-ME transplants had the high, pulsatile levels of LH characteristic of normal, ovariectomized rats. These data suggest that developing TI-DA neurons, within effective arcuate-ME transplants, became functional to reinstate or accentuate DA inhibition of prolactin secretion and, in so doing, indirectly normalized LH secretion as well.