Specific expression of a retinoic acid-regulated, zinc-finger gene, Rex-1, in preimplantation embryos, trophoblast and spermatocytes.
Specific expression of a retinoic acid-regulated, zinc-finger gene, Rex-1, in preimplantation embryos, trophoblast and spermatocytes.
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发表时间：
1991-11
期刊：
影响因子：
4.6
通讯作者：
Melissa B. Rogers;B. Hosler;L. Gudas
中科院分区：
文献类型：
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作者：
Melissa B. Rogers;B. Hosler;L. Gudas
我们之前已经分离出一个基因的 cDNA 克隆，该基因的表达因 F9 胚胎癌细胞的视黄酸 (RA) 处理而降低。核苷酸序列表明该基因Rex-1编码锌指蛋白，因此可能是转录调节因子。 Rex-1 信息水平在两个胚胎干细胞系（CCE 和 D3）中较高，而当使用四种不同的生长条件诱导 D3 细胞分化时，Rex-1 信息水平会降低。正如干细胞特异性信息所预期的那样，Rex-1 mRNA 存在于 4.5 天小鼠囊胚的内细胞团 (ICM) 中。它也存在于囊胚的极滋养层中。一两天后，在卵柱的外胎盘锥和胚胎外外胚层（滋养层衍生组织）中发现了Rex-1信息，但其丰度在直接源自ICM的胚胎外胚层中大大减少。 Rex-1在第18天的胎盘（小鼠妊娠18天）中表达，该组织主要来源于滋养层。唯一含有可检测量的 Rex-1 mRNA 的测试成人组织是睾丸。对生殖细胞缺陷型小鼠睾丸和阶段特异性生殖细胞制剂的 RNA 原位杂交和 Northern 分析表明，Rex-1 表达仅限于精母细胞（正在进行减数分裂的生殖细胞）。这些结果表明，Rex-1 参与滋养层发育和精子发生，并且是 ICM 中早期细胞命运决定研究的有用标记。
We have previously isolated a cDNA clone for a gene whose expression is reduced by retinoic acid (RA) treatment of F9 embryonal carcinoma cells. The nucleotide sequence indicated that this gene, Rex-1, encodes a zinc-finger protein and thus may be a transcriptional regulator. The Rex-1 message level is high in two lines of embryonic stem cells (CCE and D3) and is reduced when D3 cells are induced to differentiate using four different growth conditions. As expected for a stem-cell-specific message, Rex-1 mRNA is present in the inner cell mass (ICM) of the day 4.5 mouse blastocyst. It is also present in the polar trophoblast of the blastocyst. One and two days later, Rex-1 message is found in the ectoplacental cone and extraembryonic ectoderm of the egg cylinder (trophoblast-derived tissues), but its abundance is much reduced in the embryonic ectoderm which is directly descended from the ICM. Rex-1 is expressed in the day 18 placenta (murine gestation is 18 days), a tissue which is largely derived from trophoblast. The only tested adult tissue that contains detectable amounts of Rex-1 mRNA is the testis. In situ hybridization and northern analyses of RNA from germ-cell-deficient mouse testis and stage-specific germ cell preparations suggest that Rex-1 expression is limited to spermatocytes (germ cells undergoing meiosis). These results suggest that Rex-1 is involved in trophoblast development and spermatogenesis, and is a useful marker for studies of early cell fate determination in the ICM.